Authors:Teréz Márián, L. Balkay, I. Fekete, Zs. Lengyel and G. Veress
The effect of plasma glucose concentration on the cerebral uptake of [18 F]-fluorodeoxy-D-glucose (FDG) was studied in a broad concentration range in a rabbit brain model using dynamic FDG PET measure- ments. Hypoglycemic and hyperglycemic conditions were maintained by manipulating plasma glucose applying i.v. glucose or insulin load. FDG utilization (K) and cerebral glucose metabolic rate (CGMR) were evaluated in a plasma glucose concentration range between 0.5 mM and 26 mM from the kinetic constant k1, k2, k3 obtained by the Sokoloff model of FDG accumulation. A decreasing set of standard FDG uptake values found with increasing blood glucose concentration was explained by competition between the plasma glucose and the radiopharmacon FDG. A similar trend was observed for the forward kinetic constants k1, and k3 in the entire concentration range studied. The same decreasing tendency of k2 was of a smaller magnitude and was reverted at the lowest glucose concentrations where a pronounced decrease of this backward transport rate constant was detected. Our kinetic data indicate a modulation of the kinetics of carbohydrate metabolism by the blood glucose concentration and report on a special mechanism compensating for the low glucose supply under conditions of extremely low blood glucose level.
Authors:P. Hermann, Zsuzsanna Berek, G. Kriván, Krisztina Márton and Anna Lengyel
Eighty persons with haematological malignancies receiving stem cell transplantation (SCT) were examined over a 24 months period. Hyposalivation, a common complaint in patients treated by intensive chemotherapy and radiotherapy can predispose to oral candidal colonisation as well. This study was focused on correlation between the fungal colonisation of the oral cavity and the total unstimulated saliva flow rate of 80 patients with haematological malignancies before and after stem cell transplantation and in addition, on their oral health state. Despite the fact that prior to being involved in the transplantation programme, the patients were subjected to dental examination and decayed teeth were found in 20 out of 80 patients (25%). From the 2233 different oropharyngeal specimens fungi were isolated before conditioning from 16 patients (20%), and during aplasia from 19 patients (23.7%). Objective xerostomia (unstimulated total saliva flow rate = 0.1 ml/min) was detected in 28 patients (35%). Stem cell transplant patients with pretransplant mouth dryness had higher incidence of Candida albicans and other fungal colonisation than those with normal saliva secretion.
Authors:Bernadett Borda, Cs Lengyel, E. Szederkényi, J. Eller, Cs Keresztes and G. Lázár
The incidence of post-transplant diabetes mellitus and its effects on the kidney allograft function and morphology were assessed. Patients were divided into three groups according to their glucose metabolism. Risk factors for diabetes were first assessed, and then changes in renal function were checked. Morphological changes in the allografts were examined by protocol biopsies. The overall incidence of diabetes was 16%. The development of diabetes was influenced significantly by the body mass index, the body weight and the age of the recipient. The incidence of diabetes was 8.6% in patients on cyclosporine A therapy and 28.8% in those on tacrolimus (p < 0.05). As to the morphology of the kidney, a significantly higher proportion of the biopsies showed severe interstitial fibrosis/tubular atrophy (p = 0.0004) and subclinical acute rejection ( p = 0.001) in the diabetic group compared to the normal one. This clinical study has revealed that the adverse effect of diabetes on the allograft can be detected with protocol biopsy before the manifestation of a functional deterioration.
Authors:B. Nemes, A. Doros, Á. Holczbauer, E. Sárváry, P. Nagy, G. Lengyel, A. Kiss and Zs. Schaff
Hepatitis C (HCV) is one of the main causes of liver transplantation (OLT). Previously we have reported that high serum C RNA level correlates with the severity of histopathological signs and poor clinical outcome. The core antigen of virus C is known to interfere with chaperones in the hepatocytes, results in an endoplasmic reticulum (ER) stress. In this study HCV positive liver transplanted patients were evaluated, whether there are correlations among chaperone expression, recurrence and viral titer. Patients were enrolled after surviving the first month following OLT. Sera were collected regularly, and biopsies were taken on demand following OLT. The diagnosis of recurrent HCV was proven by Knodell-Ishak scoring. In this case ribavirin+interferon were initiated, and maintained for one year. All chaperones were upregulated in the transplanted liver graft showing recurrent hepatitis C disease. ATF6, GP96, GRP78, CNX and CLR chaperones were upregulated significantly compared to their levels in normal livers. Except for one chaperone, the level of upregulation did not correlate with the serum's HCV-RNA titre: the only difference between Group1 and 2 (RNA titre above and below 8.78 106 respectively) was that the level of ATF6 was 1.6 times higher in Group1 compared to Group2. The expression of all chaperones was reduced, and some even became downregulated after the interferon treatment. In accordance with the literature our results suggest that hepatitis C might induce apoptosis through ER-stress. Those cells exposed to a high C viral load, had a lower chance to be eliminated.
Authors:T. Ábel, A. Blázovics, A. Wimmer, G. Bekő, B. Gaál, B. Blazics, M. Eldin, J. Fehér, I. Szabolcs and G. Lengyel
The effect of moderate alcohol consumption on insulin sensitivity is actually a topic of intense research nowadays. In this study we investigated the effect of moderate consumption of white wine in subjects with metabolic syndrome. Thirty-two patients with metabolic syndrome participated in this prospective, randomised, double-blind study. The subjects received either Müller-Thurgau (n=14) or Pintes (n=18) sort of wine for 4 weeks. Male and female subjects consumed 300 ml wine (30 g alcohol) and 200 ml wine (20 g alcohol) per day, respectively. Clinical and laboratory parameters were determined before and after the period of wine consumption. The HOMA-IR showed a significant decrease upon the consumption of both sorts of wine as compared to the baseline (Müller-Thurgau group: 1.85±2.1 vs. 1.06±0.6; P=0.03; Pintes group: 2.28±2.04 vs. 1.08±0.6; P=0.002). Four weeks of wine consumption lead to significant decreased of ALT (alanine aminotransferase; Müller-Thurgau group: P=0.003; Pintes group: P=0.002) and AST (aspartate aminotransferase; Müller-Thurgau group: P=0.003; Pintes group: P=0.02). Epidermal growth factor (EGF) levels increased significantly in both groups (Müller-Thurgau group: P=0.004; Pintes group: P=0.001). The plasma reducing capacity increased significantly upon the consumption of both sorts of wine (Müller-Thurgau group: P=0.002; Pintes group: P=0.001). In patients consuming Müller-Thurgau there was a significant decrease of total cholesterol (5.4±1.4 vs. 4.9±1.2 mmol l−1; P=0.006) and LDL-cholesterol (3.9±1.1 vs. 3.6±1.2 mmol l−1; P=0.04) levels. In the Pintes group we found no significant difference either in total cholesterol (5.1±0.6 vs. 5.0±0.7 mmol l−1; P=0.25) or LDL-cholesterol (3.6±0.6 vs. 3.6±0.6 mmol l−1; P=0.5) concentrations. The results of our study confirm that moderate consumption of white wine increases insulin sensitivity and EGF levels in persons with metabolic syndrome.
Authors:Péter Csathó, E. Osztoics, J. Csillag, T. Lengyel, L. Gonda, L. Radimszky, G. Baczó, M. Magyar, K. R. Végh, M. Karátsonyi, T. Takács, A. Lukács and T. Németh
Depending on their origin, sedimentary phosphate
rocks (PRs) may differ in their P solubility, and, as a consequence, in their
agronomic effectiveness. The effect of six phosphate rocks (PR) - originating
from Algeria (ALG), North Florida (FLO), North Carolina (NCA), Senegal (SEN)
Morocco (MOR) and Hyperphosphate (HYP) with various P solubility (evaluated by
2% formic acid, 2% citric acid, and neutral ammonium citrate) - as well as
single superphosphate (SSP) and superphosphate + lime (SSP + Ca) (each P source
on 4 P levels, with doses of 0, 100, 400 and 1600 mg P
soil) on the shoot yield of tillering stage spring barley, soil available
P (i.e. H
O, Olsen, Bray1, Lakanen-Erviö (LE) and ammonium lactate
(AL) extractable P contents) were studied in pot experiments set up with acidic
sandy soil (Nyírlugos, Hungary) and acidic clay loam soil (Ragály, Hungary),
both with low P supplies. The average
spring barley shoot yield at the beginning of shooting was 95% higher on the
colloid-rich acidic (pH
: 4.5) clay loam soil than on the
colloid-poor acidic (pH
: 3.8) sandy soil. The differences in the
solubility of phosphate rocks showed close correlation to the differences in P
responses. On both soils, the correlation between total PR-P added and P
responses in spring barley shoot yield was much weaker than that between
neutral ammonium citrate soluble PR-P added and P responses in spring barley
shoot yield. When phosphate rocks were applied as P sources, the comparison of
soil test P methods showed a different picture on the two soils. In the case of
the acidic sandy soil (Nyírlugos), the strongly acid LE-P (r² = 0.83) and
AL-P (r² =0.74) tests gave the highest correlation coefficients with
spring barley responses to P, while on the acidic clay loam soil (Ragály) these
were achieved by the Olsen-P (r² = 0.88) and Bray1-P (r² =0.88)