The extenders and freezing rates from three different freezing protocols were combined and compared to each other in order to study the post-thawing acrosome integrity and fertility of frozen dog sperm. A commercial bovine TRIS-base extender (TRILADYL) and two self-made canine semen extenders (Norwegian and Dutch) were combined with a conventional bovine and two canine freezing regimes, and acrosome integrity of frozen/thawed spermatozoa was assessed by fluorescein isothiocyanate conjugated peanut agglutinin staining (FITC-PNA). Differences between freezing/thawing protocols were reflected in the proportion of cells with acrosomal damage and not based on motility results. It was concluded that during dog semen cryopreservation extenders had less influence on the post-thawing sperm quality than did the freezing rates. The optimal extender/freezing rate combination (TRILADYL/Norwegian) was used in the clinical practice to evaluate the fertility of frozen sperm administered by intrauterine insemination using a surgical approach. The pregnancy rate was 57% (4/7), but the average litter size was low (2.8). This may have been due to the insufficient sperm numbers contained in an insemination dose and/or to the incorrect timing of artificial insemination (AI). The final conclusion is that the commercial bovine extender is useful for freezing dog semen, and the TRILADYL/Norwegian freezing protocol is recommended as the most advantageous combination for the freezing of canine semen in the clinical practice.
An improved method for the synthesis of four heterotrinuclearpolyacids of the type: Hx[EM′yM″zO40⋅nH2O (E=P, Si; M′=Mo, W; M″=V, W) was elaborated. The studied compounds were characterized by elemental analysis, IR spectra and thermal behaviour over
20–800C temperature range.
As it is well known that due to the continuously decreasing trend of the alternative energy technologies and the increase in the costs of the traditional fossil fuels, the development of resident or commercial buildings with greatly reduced energy needs becomes a high ranked and more pressing scientific challenge. In topic with this technological and scientific provocation, the paper presents a LabView software-based building supervising and events monitor system designed and developed with the basic purpose to serve as support for net zero-energy control strategies implementation in intelligent buildings. Afterwards a brief presentation of the adopted intelligent building development strategy and the net zero-energy concept are presented and discussed. Once these theoretical aspects have been expressed and clarified, a multi-layer type implementation is unfolded, where the background layer is a building supervising and events monitor system. The main software development steps of this system are presented in details to prove the feasibility and reliability of the adopted implementation strategy.
Authors:I. Szabó, G. Bognár, L. Kereskai, K. Szász and D. Lőrinczy
Shoulder pain is a common presentation of
the dysfunction of the glenohumeral joint. The long head of the biceps tendon
has been proposed as a source of pain in rotator cuff pathologies. The rotator
cuff is a dynamic stabilizer of the glenohumeral joint, and its tear can create
different shoulder dysfunctions. The long head of the biceps has a special
intraarticular localisation, so the arthricular destruction affects its tendon
too. In the process of the rotator cuff degeneration and tear the structure
of the biceps tendon pathological transforms. With foregoing studies authors
have demonstrated the feasibility of DSC in the investigation of the musculoskeletal
The aim of this study was to establish the curves and
the histological properties of the tendon of the long head of the biceps in
different magnitudes of the rotator cuff tear on cadavers. The DSC results
clearly proved that definitive differences are present between the structural
state of the tendons in different magnitudes of the rotator cuff tears, which
have also been demonstrated by the histological examination.
Authors:B. Kádár, M. Szász, Katalin Kristóf, Natasa Pesti, G. Krizsán, Julianna Szentandrássy, L. Rókusz, K. Nagy and Dóra Szabó
The aim of the study was to investigate the biofilm-production of 60 Pseudomonas aeruginosa strains isolated from clinical samples and to examine the effect of different antimicrobials and their combinations with clarithromycin on biofilm-formation.The minimal inhibitory concentrations (MICs), minimal biofilm inhibitory concentrations (MBICs), and antibiotic synergy by calculating the fractional inhibitory concentration (FIC) index were determined for the following antibiotics: ceftazidime, cefepime, piperacillin/tazobactam, imipenem, meropenem, levofloxacin, ciprofloxacin, gentamicin, amikacin, tobramycin, netilmicin and clarithromycin.A total of 14 (23.3%) isolates out of 60 isolates of P. aeruginosa were biofilm positive. Cefepime, imipenem and meropenem had the lowest MIC90 values. Piperacillin/tazobactam and clarithromycin had the highest MIC90 values. Imipenem, meropenem, piperacillin/tazobactam and clarithromycin had the lowest MBIC90 values.For biofilm-forming P. aeruginosa strains 2-fold to 128-fold higher MBIC values than MIC values were obtained for ceftazidime, cefepime, imipenem, amikacin and netilmicin. The MBIC was 2-fold to 512-fold lower then the MIC values in the case of piperacillin/tazobactam, ciprofloxacin, levofloxacin and clarithromycin.Synergy was generally demonstrated for clarithromycin in combination with aminoglycosides, fluoroquinolones or ceftazidime. However, surprisingly it was found that combinations of clarithromycin with carbapenems or cefepime led to an antagonistic interaction: combination of clarithromycin with imipenem, meropenem or ertapenem showed antagonism in 37.5%, 50% and 62.5% of the strains tested whereas its combination with cefepime expressed antagonism in 75% of the strains, respectively. To the best of our knowledge no one has previously described this phenomenon so far.