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  • Author or Editor: G. Tóth x
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Abstract  

The separation of low molecular weight compounds labelled with125I has gained importance with the advent of radioimmunoassay (RIA) in which overwhelmingly125I-labelled analytes are used as tracers. The chloramine T labelling method proved to be the most suitable procedure to introduce radioiodine atom via aromatic electrophilic substitution either in the thyronine molecule or in the tyrosine methyl ester (TME) side chain coupled as a prosthetic group to different compounds which do not possess phenolic hydroxyl groups.

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Abstract  

3,3′-diiodothyronine, 3,3′,5-triiodothyronine, 3,3′,5′-triiodothyronine and thyroxine was labelled with125I and/or131I by the use of the chloramine T method.1.2 The labelled products were separated by adsorption chromatography using Sephadex LH-20 dextrangel as adsorbent and aqueous solution of ethanol as eluent.

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Abstract  

The distribution of mono- poly- and unlabelled protein molecules in the radiolabelling mixture was calculated from probability point of view as a function of the radioiodine/protein ratio.

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