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  • Author or Editor: Ghada M. Hadad x
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A new, simple, precise, accurate, stability-indicating, and rapid high-performance thin-layer chromatography (HPTLC) method was developed and validated for the determination of ondansetron hydrochloride in pure form and pharmaceutical formulations. The method used Merck HPTLC aluminum plates precoated with silica gel 60 F254 as the stationary phase. The mobile phase consisted of chloroform–methanol–ethyl acetate (7:2:1, v/v). This system was found to give a compact spot of ondansetron (RF value of 0.67 ± 0.011); ondansetron was subjected to acid and alkali hydrolysis, oxidation, and photodegradation. The wavelength of TLC scanner was set at 302 nm for both detection and quantitation. The calibration curves were linear over the range of 25–500 ng spot−1. The limit of detection was 4.9 ng spot−1, and the limit of quantitation was 14.7 ng spot−1. The proposed analytical method was validated according to the International Conference on Harmonization (ICH) guidelines, and the results were acceptable. The proposed method has been successfully applied to the determination of the studied drug in its pharmaceutical preparations and it gave excellent % of recovery. The results showed excellent agreement with the reported method with respect to precision and accuracy.

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The combination of itopride (ITP), pantoprazole (PAN), and mosapride (MS) is widely used in the treatment of many gastrointestinal tract (GIT) disorders. For that purpose, a new, simple, precise, accurate, and rapid high-performance thin-layer chromatography (HPTLC) method was developed and validated for the simultaneous determination of ITP, PAN, and MS in their pharmaceutical formulations. The method used Merck HPTLC aluminum plates precoated with silica gel 60 F254 as the stationary phase. The mobile phase consisted of methylene chloride–ethyl acetate–methanol– ammonia (25%) (12:2:0.8:0.2, v/v); this system was found to give compact spot of itopride (R F value of 0.22 ± 0.008), pantoprazole (R F value of 0.41 ± 0.006), and mosapride (R F value of 0.62 ± 0.029). The wavelength of thin-layer chromatography (TLC) scanner was set at 289 nm for both detection and quantitation. The calibration curves were linear over the range of 100–1500 ng spot−1 for ITP and MS, and 70–1500 ng spot−1 for PAN. The detection limits were 32.5, 16.8, and 29.8 for ITP, PAN, and MS, and the quantitation limits were 98.5, 50.3, and 90.5 for ITP, PAN, and MS. The proposed analytical method was validated according to the International Conference on Harmonization (ICH) guidelines, and the results were acceptable. The proposed method has been successfully applied for the determination of the studied drugs in their pharmaceutical preparations as well as in spiked human plasma and it gave excellent percent of recovery. The results showed excellent agreement with the reported method with respect to precision and accuracy.

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Abstract

In this study, a simple, fully validated and rapid reversed-phase HPLC with photodiode array detector method was developed for the simultaneous determination of 11 selected phenolic antioxidants over 33 min in personal care and food samples containing extracts of green apple, pomegranate (Ponica granatum) and argan oil (Argania spinosa). The method was performed using NUCLEODUR C18 column 5 µm particle size and 12.5 cm length. The HPLC mobile phase was prepared as follows, solution A: 1% aqueous acetic acid and solution B: Acetonitrile. The method was gradient at flow rate 1.0 mL/min with a simple mobile phase, minimal sample preparation, and diminished organic solvent usage (3% acetonitrile for almost 90% of the run time). The detection was carried out at 278 nm. The method presented good precision and accuracy with RSD% values ranged between 0.33 and 1.94% and wide linear range. The developed method was successfully applied on 67 personal care and food products present in Egyptian market and can be used for routine screening in laboratory for the regular quality control of the antioxidant content for products containing the mentioned extracts.

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