Authors:Nimzing G. Ladep, Oche O. Agbaji, Patricia A. Agaba, Muazu A. Mohammed, Godwin E. Imade and John A. Idoko
To determine the relationship between the immunological status and anti-HCV detection of HIV-1 infected individuals in an HIV/AIDS cohort within an African population.
Retrospective study of randomly selected HIV/AIDS patients.
The biodata of 1044 consenting HIV infected patients were analyzed retrospectively. These patients had been enrolled following the confirmation of their HIV status by western blot assay after an initial reactive ELISA. Blood obtained from the patients were subjected to serological tests to determine their anti-HCV antibody status using third generation Enzyme Immunoassay (DIA.PRO Diagnostic, Bioprobes srl, Italy). CD4 count was done by flow cytometry (Partec Cyflow, Germany) and viral load by PCR (Roche Amplicor 1.50). The data were analyzed using the Epi Info 2004 statistical software.
Ninety out of 1044 patients (8.6%) were positive for anti-HCV antibodies. The rate of HCV infection was directly proportional to the CD4+ group (6.8% vs. 15.4% for < 201 and > 800, respectively,
There is an associated higher chance of detecting anti-HCV in sera of the HIV-1 infected patients whose immunological status are better compared to severely immunocompromised ones.
Authors:Oche O. Agbaji, Nimzing G. Ladep, Patricia Agaba, Bitrus P. Badung, Monday L. Danung, Godwin Imade, John A. Idoko, Rob Murphy and Phyllis Kanki
There is a rising trend of hepatitis B co-infection among HIV positive individuals and this may negatively impact their morbidity and mortality. This study analyzed HBsAg prevalence, its demographic distribution and relationship to the immunological and viral load status of HIV positive cohort in Jos.
This was a cross-sectional study conducted at the antiretroviral treatment centre in Jos between August 2004 and April 2005. Biodata of 1042 consenting HIV positive patients were obtained. Blood samples obtained from them were tested for hepatitis B surface antigen by Enzyme Linked Immunosorbent Assay. CD4 cell count was done by flow cytometry (Partec, Germany) and viral load by PCR techniques (Roche Amplicor 1.5). The data obtained were analyzed using Epi Info 2004 statistical software.
One hundred and sixty seven (16%) of the 1042 patients were reactive to HBsAg. Thirteen (22.4%) of 58 divorced patients had the highest co-infection rate. Ninety-seven (14.2%) out of 681 females were co-infected with hepatitis B, while seventy (19.4%) out of 361 males had the co-infection. Most of the patients at the AIDS stage of the HIV infection were co-infected with hepatitis B. The median HIV viral load of HBsAg-positive patients was higher than that of non-HBV co-infected patients.
This study showed a high HBsAg prevalence among our patients with a significant male preponderance. This may have a negative impact on the CD4 recovery and viral load reduction of the patients on treatment.