Authors:H. Essafi, N. Trabelsi, C. Benincasa, A. Tamaalli, E. Perri and M. Zarrouk
The aim of the present study was to investigate the biological activities of Tunisian olive leaf extracts and to correlate these activities to their phytochemical composition. The phenolic profile of four Tunisian autochthonous cultivars Chemlali, Sayali, Neb jmel, and Meski was determined using LC/MS-MS. The antioxidant activity of olive leaf extracts was evaluated using DPPH test. The antiproliferative effect was also investigated using MTT assay. The phytochemical screening showed that phenolic content and phenolic class repartition were significantly affected by olive leaf cultivars. Twenty-one components were identified and oleurpein, luteolin 4-glucoside, luteolin 7-glucoside, and apigenin 7-glucoside were the major phenolic components. Among all extracts, Sayali exhibited the strongest antioxidant and antiproliferative activities (IC50 41.36 µg ml−1 / EC50 147.11 µg ml−1). The MTT result showed that olive leaf extract reduced MCF-7 cell viability in a dose-dependent manner. Western blot analysis demonstrated that olive leaf extracts exhibited antiproliferative activity through apoptosis induction.