The isotopic exchange of35S between thiosulfate/sulfite and tetrathionate/thiosulfate is examined. The kinetic data are determined. In both cases the
reactions are of second order. As the isotopic exchange between tetrathionate/thiosulfate is very fast, these reaction components
are used in high dilution. Furthermore, the extent of exchange during the separation is determined.
Outer and inner35S-labelled S3O
are prepared and then hydrolyzed at different temperatures. The separation of the reaction mixture is made by high-voltage
paper ionophoresis. The amount of activity is determined directly on the paper by liquid scintillation. The ratios of activity
of the reaction products are compared with those calculated on the basis of three different reaction mechanisms. All experimental
results agree very well with the authors' proposed reaction mechanism via the unsymmetrical intermediate−O3S−SOH. The influence of the isotope exchange between S2O
on the ratio of activity is examined at different temperatures. Attempts have been made also to capture the SO
which orginates from the first step of the S4O
Degradation by silver permits determination of the distribution of activity inside the labelled molecule for thiosulfate and
for the polythionates. When single salts are present, the reactions can be carried out either in solution or on the pherogram.
In comparison, for mixtures of sulfur anions, it is necessary to perform previously a high voltage inonophoretic separation.
The cutout zone of a definite anion is put on a new pherogram and the degradation by silver is carried out. The results correspond
within the accuracy of measurement to the theoretical values.
The isotopic exchange of sulfur-35 between trithionate/sulfit and tetrathionate/pentathionate is examined. High voltage paper
ionophoresis is applied for the separation of the reaction mixture. The amount of activity of the reaction products is determined
at different times. This enables to calculate the kinetical data of those exchange systems.