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  • Author or Editor: Harsha Tambe x
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A normal-phase high-performance thin-layer chromatographic (HPTLC) method has been established for quantitative estimation of itopride hydrochloride, a gastroprokinetic agent, in its pharmaceutical formulation and in the bulk drug substance. Analysis was performed on silica gel 60F 254 TLC plates with methanol-ethyl acetate-toluene-triethylamine, 1.0 + 2.5 + 6.0 + 0.5 ( v/v ), as mobile phase. Detection and quantitation were performed densitometrically at 1 = 230 nm. Diltiazem hydrochloride was used as the internal standard. Response to itopride hydrochloride was found to be linear in the concentration range 50.00 to 2000.00 μg mL −1 . The method was validated for accuracy and precision. Accuracy was checked by conducting recovery studies; the average recovery was found to be 99.27%. The method is simple, rapid, and precise and can be used for routine quality control.

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A normal-phase high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous quantitative determination of diclofenac sodium and paracetamol in a pharmaceutical formulation and in bulk drug powder. The analysis was performed on silica gel 60F 254 HPTLC plates with toluene-ethyl acetate-methanol-formic acid, 5.0 + 4.0 + 1.0 + 0.01 ( v / v ), as mobile phase. Detection and quantitation were performed densitometrically at λ = 260 nm. Aceclofenac was used as the internal standard. Responses of diclofenac sodium standard and paracetamol standard were linear functions of concentration in the ranges 30–800 ng μL −1 and from 300–2000 ng μL −1 , respectively. The method was validated to determine its accuracy and precision. Accuracy was checked by conducting recovery studies; average recovery from the pharmaceutical preparation was 99.57% for diclofenac sodium and 100.51% for paracetamol. This HPTLC method developed for simultaneous quantitative determination of diclofenac sodium and paracetamol in pharmaceutical preparations is simple, rapid, and precise and can be used for routine quality control. The method was also used to determine the amounts of diclofenac sodium and paracetamol in a mixture of their respective bulk drug powders.

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