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  • Author or Editor: Hermann Buerstmayr x
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The aim of the project is the detection, genetic mapping and characterization of quantitative trait loci (QTL) that confer resistance against Fusarium head blight in a resistant line of Triticum dicoccum .

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Based on published results in 52 peer reviewed articles QTL for FHB resistance are summarized and compared. Suggestions for resistance breeding are given.

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Cereal Research Communications
Authors: Barbara Steiner, Katharina Schieszl, Ewelina Litwicka, Harald Kurz, Marc Lemmens, Haiyan Jia, Gary Muehlbauer, and Hermann Buerstmayr

Molecular mapping led to the identification of two major Fusarium head blight (FHB) resistance QTL, Fhb1 and Qfhs.ifa-5A . The actual function of the resistance genes is still unknown. The resistant line CM82036, the susceptible line Remus and six closely related lines were analyzed for differential gene expression after Fusarium attack. The related lines show contrasting levels of FHB resistance due to the possession of Fhb1 and Qfhs.ifa-5A . At anthesis plants were challenged by Fusarium graminearum or water and at six time points after inoculation gene expression of specific wheat floral tissue was analyzed by cDNA-AFLPs and the Affymetrix Wheat GeneChip.0.44% of the analyzed gene tags by cDNA-AFLPs displayed differential expressions after Fusarium attack depending on the genotype. Five of the gene tags were associated with the FHB resistance level of the genotypes and the possession of resistance alleles at Fhb1 and Qfhs.ifa-5A . These gene tags show homologies to a UDP-glucosyltransferase, wheat phenylalanine ammonia-lyase, DnaJ-like protein, pathogenesis-related family protein and a rice cDNA clone with unknown function. A thorough comparative analysis with the data gained by the Wheat GeneChip experiments is in progress.

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Cereal Research Communications
Authors: Marc Lemmens, Andrea Koutnik, Barbara Steiner, Hermann Buerstmayr, Franz Berthiller, Rainer Schuhmacher, Frank Maier, and Wilhelm Schäfer

Our main goal in this contribution was to investigate whether Fhb1 also governs resistance towards NIV, a trichothecene structurally related to DON. Here we report preliminary results on a first series of experiments done to clarify this issue. Using mutants with a disrupted trichodiene synthase, it is confirmed that NIV and DON production are fungal virulence factors important for the spread of symptoms in the wheat ear but not for the induction of Fusarium head blight. Both toxins affect the probability that symptoms spread. It is demonstrated that NIV is phytotoxic on wheat ears and that the purified toxin can induce symptoms identical to those described for DON. Fhb1 protects the wheat line against both NIV and DON. The mechanism of NIV resistance is not known but is probably different from the detoxification mechanism of DON. We could not confirm that Fhb1 increases resistance to both NIV- and DON-producing Fusarium strains.

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