Authors:Karin Huber, Maria Buerstmayr, and Hermann Buerstmayr
The aim of the project is the detection, genetic mapping and characterization of quantitative trait loci (QTL) that confer resistance against
head blight in a resistant line of
Authors:Barbara Steiner, Katharina Schieszl, Ewelina Litwicka, Harald Kurz, Marc Lemmens, Haiyan Jia, Gary Muehlbauer, and Hermann Buerstmayr
Molecular mapping led to the identification of two major Fusarium head blight (FHB) resistance QTL,
. The actual function of the resistance genes is still unknown. The resistant line CM82036, the susceptible line Remus and six closely related lines were analyzed for differential gene expression after Fusarium attack. The related lines show contrasting levels of FHB resistance due to the possession of
. At anthesis plants were challenged by
or water and at six time points after inoculation gene expression of specific wheat floral tissue was analyzed by cDNA-AFLPs and the Affymetrix Wheat GeneChip.0.44% of the analyzed gene tags by cDNA-AFLPs displayed differential expressions after Fusarium attack depending on the genotype. Five of the gene tags were associated with the FHB resistance level of the genotypes and the possession of resistance alleles at
. These gene tags show homologies to a UDP-glucosyltransferase, wheat phenylalanine ammonia-lyase, DnaJ-like protein, pathogenesis-related family protein and a rice cDNA clone with unknown function. A thorough comparative analysis with the data gained by the Wheat GeneChip experiments is in progress.
Authors:Marc Lemmens, Andrea Koutnik, Barbara Steiner, Hermann Buerstmayr, Franz Berthiller, Rainer Schuhmacher, Frank Maier, and Wilhelm Schäfer
Our main goal in this contribution was to investigate whether
also governs resistance towards NIV, a trichothecene structurally related to DON. Here we report preliminary results on a first series of experiments done to clarify this issue. Using mutants with a disrupted trichodiene synthase, it is confirmed that NIV and DON production are fungal virulence factors important for the spread of symptoms in the wheat ear but not for the induction of Fusarium head blight. Both toxins affect the probability that symptoms spread. It is demonstrated that NIV is phytotoxic on wheat ears and that the purified toxin can induce symptoms identical to those described for DON.
protects the wheat line against both NIV and DON. The mechanism of NIV resistance is not known but is probably different from the detoxification mechanism of DON. We could not confirm that
increases resistance to both NIV- and DON-producing Fusarium strains.