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The p53 tumour suppressor plays central role in the maintenance of genome integrity. P53 deficient fruit flies are highly sensitive to ionizing radiation (IR) and show genome instability suggesting that the Drosophila melanogaster p53 ( Dmp53 ) is necessary for the proper damage response upon IR. We found that Dmp53 null fruit flies are highly sensitive to ultraviolet radiation (UV) as well. We analyzed the expression levels of apoptotic genes in wild type and Dmp53 null mutant animals after UV or IR using quantitative real-time RT-PCR. Ark (Apaf-1related killer) was induced in a Dmp53-dependent way upon UV treatment but not by IR, hid ( head involution defective/ wrinkled ) was induced upon both types of DNA damage, while reaper was induced only upon IR but not UV treatment. Using microarray analysis we identified several further genes that are activated upon UV irradiation in the presence of wild type Dmp53 only. Some but not all of these genes show Dmp53-dependent activation upon IR treatment as well. These results suggest that Dmp53 activates distinct cellular pathways through regulation of different target genes after different types of DNA damage.

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The paper presents significant aspects regarding the concept and details of constructive solutions used to design and implement an energy efficient school building in Romania. Although the site is in a moderate seismic zone, the structural needs of the building impose specific insulation solutions for making the passive house concept applicable. Renewable energy sources are also applied to optimize the maintenance cost and to reduce the payback period to a feasible level. In order to prove the benefits of the solutions, to justify the higher initial investment, to improve the real-time energy consumption of the building, as well as to keep track of the internal climate parameters, a complex monitoring system was planned and implemented. Details and correlation between specific details and temperature sensors distribution are also presented.

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Soy is increasingly used as a food additive. In women, it is recommended as an alternative to hormonal replacement therapy and/or a preventive agent against breast cancer and osteoporosis. Previous data revealed that rats fed on raw soybean diet developed pancreas hypertrophy and hyperplasia. An animal model was used in our experiment to examine the effects of raw soybean on parotid gland of rats. The purpose of this study was to light on the role of different neuropeptide-containing nerve fibres on changes in the acinar cells. The morphological structure and the neuropeptide-containing nerve fibers (NPY, GAL, SOM, SP, CGRP, VIP) of the glands were examined by light and electronmicroscopy. Significant increase of the organ weight was detected in the animals fed by raw soybean compared to control samples. Changes in the number of different neuropeptide-containing nerve fibres were various: Significant decrease in the NPY-immunoreactive (IR) and significant increase in the GAL-IR nerve fibres were observed. Slight but not significant increase in VIP-IR; and no changes in the other IR nerve fibres were found. The electronmicroscopic alterations of acinar cells were manifest, where a large number of undifferentiated glandular cells were seen among the acini. Some of these cells contained two nuclei and their cytoplasm contained only a few secretory granules. These granules were similar to those in the mucous cells but not to the serous ones. The results presented here provide direct morphological evidence for the role of raw soy on the density of different neuropeptide-containing nerve fibres inducing proliferation in the acinar cells of parotid glands from rats. It is suggested that the hypertrophic changes in the glands might be caused by the alterations of nerve fibres.

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Abstract

Common faint and the so-called situational syncope are usually listed together in the category of “neurally mediated syncope”. Although vasovagal syncope has been subject of extensive research in the past years, its mechanism is not fully understood. Much less information is available about situational syncope. Numerous entities in this heterogeneous group are related to excessive straining (e.g. weight lifter's or trumpet player's syncope). Cough syncope is also associated with tremendous intrathoracic, intravascular, and even intracranial pressure fluctuations. Although reflex responses are certainly triggered during cough, their relative role, and their analogy with the classical vasovagal reaction are not clear. We report a case of vasovagal syncope and cough reaction occurring in the same subject during laboratory testing.

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Abstract  

The reaction of the nca [18F]fluoride ion was investigated toward a series of N6-benzoyl 2",3"-isopropylidene-adenosine-5"-sulfonates including the methane-(mesylate), p-toluene-(tosylate), p-nitro-benzene-(nosylate)- and 2,2,2-trifluoro-ethane-sulfonate (tresylate) derivatives under usual nucleophilic substitution conditions. In these reactions cyclisation of the title compounds was observed whilst the radiofluorination took place only with low yield. The fluorine-18 uptake was found to be 1.17% for mesylate, 1.46% for tosylate, 0.99% for nosylate and 0.40% for tresylate under the conditions applied.

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Abstract

The recycling of greywater for non-potable water applications, such as toilet flushing or irrigation, is a potential solution to reduce potable water consumption. To estimate the quality of raw greywater samples, we measured several physical, chemical and biological parameters. We used Hygiene Monitor test kits to determine microbiological presence in the samples. The greywater samples showed a high variability for all the measured parameters. In general, we found that greywater sources had lightly alkaline character with a considerable inorganic and organic content. About half of the analyzed samples can be characterized as highly contaminated water source with organic substance. The average BOD5 and DOC values of greywater samples were 429 ± 410 mg l−1 and 341 ± 348 mg l−1. The samples showed high similarity concerning the total plate counts parameter (range from 5 to 6 log10 cfu ml−1). Among the three greywater sources, the samples from dish sources showed the lowest coliform content (3.9 log10 cfu ml−1) and the bath samples were contaminated with coliforms to the highest degree (4.9 log10 cfu ml−1). The results of E. coli contamination tests proved that all greywater sources can be contaminated with pathogen bacteria, consequently the treatment before reuse of greywater is indispensable.

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Abstract

Several small and big water streams cut across Hungary; the protection of these water resources is in our common interest. To perform this protection, it is necessary to carry out extensive condition surveys of these surface waters. Unfortunately, our surface water streams are not properly investigated; it is true especially for the small ones. That is why we started to study the water quality of Nagy Brook, which is loaded by many point sources of pollution. During our work we have done measurements on spot and in laboratory, as well. In this paper we are presenting only those results which were measured on the spots along the Nagy Brook. Our results demonstrate how large the effect of inflowing treated wastewater is on the quality of a seasonal water stream and how the water level affects the studied parameters and what kind of the chemical and ecological water conditions can be developed under these circumstances.

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Abstract  

5-Deoxy-5-[18F]fluoro-adenosine was synthesised by nucleophilic radiofluorination reactions of 5-deoxy-5-haloadenosines. The homogeneous isotope exchange in 5-deoxy-5-fluoro-adenosine was also investigated. The conversion of these reactions was found to be rather low and depends on the strength of the halogen-carbon bond: 0.248% for chloride-, 0.488% for bromide- and 1.070% for iodide-derivative; there was no reaction observed in the case of fluoro-compound.

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Journal of Radioanalytical and Nuclear Chemistry
Authors: I. Boros, G. Horváth, S. Lehel, T. Márián, Z. Kovács, J. Szentmiklósi, G. Tóth, and L. Trón

Abstract  

[11C]-labeled form of ten A2a adenosine receptor specific 8-styryl-7-methyl-xanthine derivatives ([11C]-caffeines) were synthesised by N-methylation of the corresponding 8-styryl-xanthine derivatives using [11C]-methyl iodide in optimized reaction conditions. The results show that the [11C]-methylations take place with excellent radiochemical yields (35–93%), and can be utilised easily in online preparations. These labeled ligands may facilitate the positron emission tomographic (PET) investigation of adenosine A2a receptors.

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Using yeast two-hybrid screens we determined that Drosophila (Dm)p53 interacts with proteins involved in sumoylation (UBA2, UBC9 and PIAS) through different regions of its C-terminal domain. A K302R point mutation within a single canonical sumoylation site of Dmp53 did not abolish the observed interactions. These observations prompted us to analyze whether Dmp53 sumoylation at this site has any functional role in vivo. Genetic assays showed that deleting one copy of genes involved in sumoylation (lwr, Su(var)2–10 or smt3 heterozygosity) enhanced slightly the mutator phenotype of Dmp53. We compared the in vivo effects of wild type and K302R Dmp53 overproduced from transgenes and determined that similar levels of expression of the mutant and wild type proteins resulted in similar phenotype, and the two proteins showed similar cellular localization. The half life and the trans-activator activity of K302R mutant and wild type Dmp53 were also comparable. Lastly, by analyzing wild type and K302R Dmp53 expressed at different levels in animals and in S2 cells we detected no differences between the mobility of the mutant and wild-type protein. From these data we conclude that under normal developmental conditions the loss of SUMO modification at K302 does not affect Dmp53 function significantly.

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