In this study, antioxidant, oxidant, antimicrobial, and antiproliferative activities of Asparagus acutifolius L. and Asparagus officinalis L., known for their nutritional properties, were determined. In this context, methanol (MeOH) and dichloromethane (DCM) extracts of plants were obtained. Total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were determined using Rel Assay kits. Antimicrobial activities of plant extracts were determined against the test microorganisms using the agar dilution method. Antiproliferative activity was tested on the lung cancer cell line A549. As a result of the studies, it has been determined that the plant species have high antioxidant potential. In addition, it was observed that the antifungal potentials of plant extracts are high. Antiproliferative activity was determined to be at high level in both plant species. As a result, it has been determined that A. acutifolius and A. officinalis have medical potential and can be used as natural agents in pharmacological designs.