This study investigated whether kisspeptin-10 (KP-10) prevents diabetic rhesus monkeys from insulin-induced hypoglycemic shock.
Materials and methods
Thirty-six adult male rhesus monkeys were used, six in each group. Diabetes was induced with streptozotocin (45 mg/kg b.w.; single dose i.v.). Groups were as: saline control, insulin alone, pre-insulin (treated with KP-10, 30 min before insulin), post-insulin (treated with KP-10, 30 min after insulin), treated with premix dose of KP-10 (50 μg) and insulin, and the group treated with the kisspeptin antagonist P234 (50 μg). Following an overnight fast, each animal was subjected to respective treatment, and blood glucose concentrations were recorded every 30-min interval for 3 h.
Intergroup comparisons demonstrated that treatment with KP-10 prior to insulin administration and kisspeptin–insulin premix treatment allowed blood glucose levels to rise to significantly higher levels (p < 0.001) by 180 min in diabetic and healthy animals compared to treatment with insulin alone. However, intragroup comparisons revealed a significant decrease in blood glucose level in diabetic animals only. Treatment with P234 antagonist followed by insulin administration abolished the preventive action of kisspeptin, whereby blood glucose decreased significantly (p < 0.001) in both diabetic and healthy animals. KP-10 post-insulin treatment, however, remained ineffective and led, instead, to significantly decreased glucose concentrations by 180 min in both diabetic and healthy animals when compared to animals treated with insulin alone.
KP-10 bears therapeutic potential to prevent hypoglycemic shock that may sometimes occur during intensive insulin therapy. Several pharmacological aspects of its interaction with insulin and other drugs, however, remain to be investigated.
Authors:J. Zaidi, M. Arif, I. Fatima, S. Ahmed, and I. Qureshi
Rapid radiochemical neutron activation analysis (RNAA) procedures were developed and employed for the determination of 32
trace impurities in high purity aluminum thin foils. Anion exchange column chromatography was developed for the sequential
group chemical separation of various elements which helped in reducing the spectral interferences and improving the sensitivity
of the method. The procedure is simple and requires a very short time to separate the elements in three groups for radiometric
assay. To determine very low contents of uranium and thorium,239Np and233Pa as activation products were separated using anion exchange and coprecipitation methods. The impurity contents were found
to be low, therefore, their adverse effects on microelectronic devices would be negligible. Our data could partially be compared
with the data reported in literature.
Authors:J. Zaidi, M. Arif, I. Fatima, S. Ahmad, and I. Qureshi
Extensive use of chewing gums, by children in particular, entails the evaluation of trace element contents in them. Radiochemical neutron activation analysis (RNAA) was successfully employed to determine the concentration of 35 trace elements (essential, toxic and nonessential) in eight different brands of chewing gum generally consumed in Rawalpindi/Islamabad area. Comparison of trace element data of our work with literature has been presented. None of the elements detected in the brands of chewing gum examined was found to be present at a level representing a substantial contribution to the total dietary intake of the element.
Authors:J. Zaidi, M. Arif, I. Fatima, and I. Qureshi
Extensive use of pan, by one-tenth of world"s population, entails the evaluation of trace element contents in its ingredients. Radiochemical neutron activation analysis (RNAA) was developed and successfully employed to determine the concentration of 36 trace elements (essential, toxic and nonessential) in its four basic ingredients, leaf of betel pepper, betel nut, catechu and lime. The radiochemical separation methodology has significantly improved the detection limits of most of these elements due to suppression of Compton background. This study provides the base-line values of certain toxic and essential elements in these ingredients. The daily intake of essential and toxic elements through pan was estimated and compared with the recommended values. The cumulative intake of Mn is four times higher than the recommended value and that of toxic elements is well below the tolerance limits.
Authors:J. Zaidi, I. Qureshi, M. Arif, and I. Fatima
Neutron activation analysis was used for the determination of 29 trace impurities is high-purity semiconductor grade Ge and Si. In order to determine very low contents of uranium and thorium,239Np and233Pa activation products were separated using anion exchange and LaF3 coprecipitation methods. The impurity contents were found to be very low, and therefore their adverse effects would be negligible.
Authors:A. Mannan, S. Waheed, I. Fatima, and I. Qureshi
A non-destructive neutron activation analysis technique has been developed for the determination of hafnium and zirconium in low grade uranium ores. In order to calculate the fission contribution of235U, thermal neutron absorption cross-section /
a/ for94Zr has been determined. The study shows that 1 g of uranium produces the same activity as from 10.03 g of zirconium. Based on this fact, the degree of interferences have been calculated for each sample and the necessary corrections have been applied. The values have been compared with the reported IAEA and NBS values.
Authors:J. Zaidi, I. Qureshi, M. Arif, and I. Fatima
IAEA and NBS biological certified reference materials have been analyzed by instrumental neutron activation analysis and concentration of 17 elements was determined. The elements determined were either completely missing or their certified values were not given in the original compilations. For quality assurance of our work, a comparison of data on missing trace elements in some of the CRMs has been presented. It seems that second round of analysis for the certification of these elements would be useful.
Authors:J. H. Zaidi, I. Fatima, M. Arif, and I. H. Qureshi
Extensive use of coffee, by one-third of world's population, entails the evaluation of trace element contents in it. Instrumental
neutron activation analysis (INAA) was successfully employed to determine the concentration of 20 trace elements (essential,
toxic and nonessential) in four samples of coffee beans of various origins and two instant coffee brands most commonly consumed
in Pakistan. This study provides the base-line values of certain toxic and essential elements in coffee. The daily intake
of essential and toxic elements through coffee was estimated and compared with the recommended values. The cumulative intake
of Mn is four times higher than the recommended value and that of toxic elements is well below the tolerance limits.
Authors:M. Wasim, J. Zaidi, M. Arif, and I. Fatima
The k0 method has been introduced at the 30 kW miniaturized neutron source reactor (MNSR) at the Pakistan Institute of Nuclear Science
& Technology (PINSTECH). It involved the full energy peak efficiency calibration of the HPGe detector for different counting
geometries and the characterization of the neutron flux at four inner irradiation channels. The latter involved the determination
of the thermal to the epithermal flux ratio, epithermal flux shape factor, the modified spectral index, Westcott’s g-factor,
the Maxwellian neutron temperature and the fast flux. The method was validated by analyzing IAEA-SL1 (Lake Sediment) and NIST-SRM-1572
(Citrus Leaves) reference materials. All calculations were performed in Excel, including the optimization step. The results
revealed that most of the elements were estimated with less than 10% relative deviation from the certified value.
Authors:S. Waheed, N. Siddique, M. Arif, I. Fatima, N. Khalid, S. Rahman, M. Daud, and M. Wasim
This paper presents the results of the proficiency test exercise conducted in Pakistan for the determination of trace elements
in mushroom reference material. Thirteen laboratories from different organizations of the country submitted trace elemental
data on Al, As, Br, Ca, Cd, Ce, Cl, Co, Cr, Cs, Cu, Fe, Hg, K, Li, Mg, Mn, Na, P, Pb, Rb Sc, Si, Sm, Sr, Th, Zn. Results for
Al, As, Br, Ca, Cd, Cl, Co, Cr, Cs, Cu, Fe, K, Mg, Mn, Na, Rb Sc, and Zn, in the mushroom material were reported by two or
more participating laboratories and could be subjected to statistical evaluation. The original data of these trace elements
was subjected to a computer program “Histo Version 2.1” provided by IAEA. The four outlier tests, i.e., Dixon, Grubbs, skewness
and kurtosis were applied to the data sets. Consensus (overall) mean values, absolute standard deviation, relative standard
deviation, standard error, median and range of values for these elements have been reported at a significance level of 0.05.