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  • Author or Editor: I. Polišenská x
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The deoxynivalenol content, presence of kernels infected by Fusarium spp. pathogens and the frequency of visually scabby kernels in wheat intended for human consumption were monitored in 2003–2005. About 1000 samples provided by growers from various regions of the Czech Republic were analysed each year. Immunochemical assay ELISA-based kits for the analysis of deoxynivalenol content were employed. In 2003, 2004 and 2005, deoxynivalenol was detected at the maximum levels of 5090 μg.kg −1 , 18300 μg.kg −1 and 4437 μg.kg −1 , respectively. Significant correlations were found between the deoxynivalenol content and percentage of kernels infected by Fusarium spp. pathogens, and percentage of visually scabby kernels. In all years under study, the relationship between the deoxynivalenol content and percentage of visually scabby kernels was closer than that between the deoxynivalenol content and percentage of kernels infected by Fusarium spp.

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Cereal Research Communications
Authors: P. Matušinsky, M. Váňová, L. Tvarůžek, I. Polišenská, M. Janeček and V. Smutný

Fusarium head blight caused by a complex of Fusarium species is widespread across the world and ranks among the most serious diseases in cereals. Long-term field experiments were set up to evaluate the effects of preceding crop and soil management methods on Fusarium mycotoxin (DON, deoxynivalenol) contamination of winter wheat and spring barley grain. Winter wheat and spring barley were cultivated at two locations in the Czech Republic (A: Ivanovice na Hané during 2002–2014, and B: Žabčice during 2007–2014) with preceding crops (A) alfalfa, maize, and pea; and (B) alfalfa (only for wheat), sugar beet (only for barley), and maize. Different soil management methods also were used: (A) 22 cm tillage, 15 cm tillage, 10 cm chisel, and direct drilling; and (B) 22 cm tillage, 10 cm chisel, and direct drilling. Mycotoxin content in harvested grain was analysed using ELISA. At both locations in the experiments with both wheat and barley, year had a significant effect on mycotoxin content in grain. Preceding crop was another significant factor in wheat experiments at both locations, with DON content in grain higher with maize as the preceding crop than in the cases of other preceding crops. Soil management method had a significant effect only on mycotoxin content in wheat grain grown at Žabčice, and the highest DON content was determined in the chisel variant, in which case a large amount of harvest residue remained on the soil surface or was only partially incorporated.

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