Greenhouse plastic contaminations in agricultural soils were studied to quantify and examine the macroplastic and microplastic contaminants on the soil surface, soil profile, and groundwater under greenhouse farmland. Random sampling was used to select three areas in a greenhouse farm where macroplastic and microplastic data were collected. Four composite samples were collected from shallow (0–20 cm) and deep (20–40 cm) soils for each sampling point, respectively. Three soil profiles were dug, and samples were collected at intervals of 20 cm. Groundwater samples were also collected from the same profiles at a depth of 100 cm. Microplastics were extracted using predigestion of organic matter with 30% H2O2 and density separation with ZnCl2. The total mass of macroplastics in the greenhouse farmland was 6.4 kg ha–1. Polyethylene and polyvinyl chloride were the dominant plastic structures, and the dominant sizes were 1–5 and 0.5–1.0 cm, respectively. Overall, the average abundance of microplastics in the greenhouse soil was 225 ± 61.69 pieces/kg, and the dominant size structure was 2–3 mm. The average microplastic concentrations at depths of 0–20 and 20–40 cm were 300 ± 93 and 150.0 ± 76.3 pieces/kg, respectively. The average microplastic concentration in the groundwater was 2.3 pieces/l, and fibers were the dominant plastic structure. Given that microplastics were found in greenhouse soil, soil profiles, and groundwater, we recommend the careful cleaning and disposal of plastics on greenhouse farmland and further research to shed light on the level of microplastic contamination in the soil profiles and groundwater.