Vizsgálataink során meghatároztuk a kukorica szemtermésének gyapottok-bagolylepke okozta beltartalom- és fehérje-összetétel változását. Kontroll, részkárosított és károsított minták szárazanyag-, nyerszsír-, nyersfehérje- és keményítő tartalmát hasonlítottuk össze, illetve vizsgáltuk az egyes csoportok fehérje-összetételének alakulását is. Vizsgálati eredményeink a károsítás hatására fellépő fokozott vízvesztést, kényszerérést nem igazolták. A károsítás hatására az 1 hektárra kalkulált nyerszsír (veszteség: 9,29 kg, 2,92%) és a keményítő (veszteség: 214,03 kg, 3,55%) tartalom csökkenése re-gisztrálható volt. Az ezzel párhuzamosan jelentkező nyersfehérje tartalom emelkedésének háttérében álló fehérjestruktúra változást az SDS PAGE vizsgálatok igazolták, amelyet a legkifejezőbben a károsított magvaknál a kis molekulatömegű zein fehérjék eltűnése magyaráz. A fehérjetartalom növekedésének hátterében a 114, 50, 46, 35 kDa molekulatömegű fehérjék mennyiségi növekedése áll.
Climate change has brought about an increasing level of seedcorn maggot (Delia platura Meigen, 1826) (Diptera: Anthomyiidae) damage in Hungary. In order to have a more accurate understanding of the effects of these plant injuries induced by the larvae of D. platura, the nutrient content of soybean (Glycine max L. Merill.) was studied. Our results show that the moisture, raw fat, raw fibre, and raw ash content of the batches damaged by D. platura were significantly less in comparison with that of the control samples. In response to the deleterious effect of the insect, the infected soybean plants showed forced ripening (P = 0.004) (P > 0.05). The difference of moisture content between damaged and control samples was 2.30% on average. The fact of nutritional value loss was also reflected by the alteration of sugar mobilisation. As the result of this experiment the sucrose breakdown to glucose and fructose during the germination was significantly slower in the damaged seeds than that of the control ones. Overall, this late and surprising damage caused by D. platura led to the forced ripening of the affected soybean plants and a significant change in their nutritional values. Based on the herein reported results, it is presumable that in cases when the current climatic extremities, which are envisaged to occur more frequently in the future, and effects of agricultural practices will be coincided in the future a qualitative change of the produced soybean batches can be expected through the damage caused by this fly species.
Several studies have described high correlation of salivary and blood lactate level during exercise. Measuring the effectiveness and intensity of training, lactate concentration in blood, and lately in saliva are used.The aim of our study was to evaluate the correlation between the concentration and timing of salivary and blood lactate level in endurance athletes and non-athletes after a maximal treadmill test, and to identify physiological and biochemical factors affecting these lactate levels.Sixteen volunteers (8 athletes and 8 non-athletes) performed maximal intensity (Astrand) treadmill test. Anthropometric characteristics, body composition and physiological parameters (heart rate, RR-variability) were measured in both studied groups. Blood and whole saliva samples were collected before and 1, 4, 8, 12, 15, 20 min after the exercise test. Lactate level changes were monitored in the two groups and two lactate peaks were registered at different timeperiods in athletes. We found significant correlation between several measured parameters (salivary lactate — total body water, salivary lactate — RR-variability, maximal salivary lactate — maximal heart rate during exercise, salivary- and blood lactate −1 min after exercise test). Stronger correlation was noted between salivary lactate and blood lactate in athletes, than in controls.
Saccharomonospora azurea SZMC 14600 is a member of the family Pseudonocardiaceae exclusively used for industrial scale production of primycin a large 36-membered non-polyene macrolide lactone antibiotic belonging to the polyketide class of natural products. Even though maximum antibiotic yield has been achieved by empirically optimized two-step fermentation process, little is known about the molecular components and mechanisms underlying the efficient antibiotic production. In order to identify differentially expressed proteins (DEPs) between the pre- and main-fermentation stages of primycin, comparative 2D-PAGE experiments were performed. In total, 98 DEP spots were reproducibly detected, out of which four spots were excised from gels, and identified through MALDI-TOF/TOF mass spectrometry. Peptide mass fingerprint analysis revealed peptide matches to HicB antitoxin for the HicAB toxin-antitoxin system (EHK86651), to a nucleoside diphosphate kinase regulator ((Ndk; EHK81899) and two other proteins with unknown function (EHK88946 and EHK86777).