Authors:Cheruthazhakkat Sulaiman, Velliyur Gopalakrishnan and Indira Balachandran
Phytochemical studies were carried out in different parts of three Acacia species viz. A. catechu, A. nilotica and A. leucophloea, using chromatographic and spectrophotometric analyses. Various extracts like absolute alcohol, aqueous alcohol, hydroalcohol, and water extract, were compared both qualitatively and quantitatively. The different extracts showed that they are rich sources of phenolic compounds. The various parts, such as bark, heartwood, and leaf of A. catechu and A. nilotica, showed almost similar chemical profiles.
Authors:Mundengara Deepak, Cheruthazhakkat Sulaiman, Indira Balachandran and Kitharathu P. Subhash Chandran
Canscora perfoliata Lam., an important, traditionally used medicinal plant, belongs to the Gentianaceae family. Many pharmacological activities are reported for this plant; however, the major chemical constituents are not yet explored. The present study using chromatographic techniques led to the isolation of an active xanthonoid mangiferin from the whole plant. The structural identification was carried out by spectroscopic methods, such as ultraviolet (UV), Fourier transform infrared (FTIR), mass (MS) and nuclear magnetic resonance (NMR) spectroscopy. A modified high-performance thin-layer chromatographic (HPTLC) method was developed for the quantification of mangiferin in hydroalcoholic extract. The method was validated as per the International Conference on Harmonization (ICH) guidelines. Separation was achieved on silica gel 60 F254 HPTLC plates using ethyl acetate-methanol-formic acid-acetic acid-water (10:1:1:1:1, V/V). Detection and quantification were performed by densitometric scanning at 254 nm. Linearity for the compound was observed between 100-500 ng per spot (r2=0.9979). The limit of detection and limit of quantification were 30 and 50 ng for mangiferin. The relative standard deviation for instrumental precision, intra-day precision, and inter-day precision were less than 1%. The percentage of average recovery was 97.7, indicating good reproducibility. The linear regression analysis data showed good linearity, repeatability, accuracy and specificity. The amount of mangiferin obtained from the extract was 1.4 ± 0.1% (w/w). This method can be used for routine quality control analysis and for the identification of the plant.