Natural phenolic compounds, e.g. ellagitannins, gallotannins, and flavonoid glycosides, from the aerial parts of
) have been isolated and identified. The structures of these compounds were determined by conventional methods of analysis and confirmed by MS and NMR spectral analysis. The distribution of tannins and flavonoids in
E. ciconium, E. cicutarium, E. manescavi
were examined by TLC on unmodified silica gel and on silica gel chemically modified with polar and nonpolar groups (HPTLC Si 60, HPTLC Si 60 LiChrospher, HPTLC diol, HPTLC CN, and HPTLC RP-18W).
Authors:Izabela Fecka, Adam Kowalczyk, and Wojciech Cisowski
Caffeetannins and flavonoid glycosides have been analyzed in polar solvent extracts from leaves of
Mentha piperita (Lamiaceae)
. Peppermint extracts and individual compounds isolated from the leaves were investigated by TLC with horizontal development on unmodified silica gel (HPTLC Si 60, HPTLC LiChrospher Si 60) and on silica gel chemically modified with polar (HPTLC NH
, HPTLC CN) and non-polar groups (HPTLC RP-18W). The mobile phase acetone-acetic acid, 85 + 15 (
), enabled successful separation on the aminopropyl adsorbent; water-methanol, 60 + 40 (
), was best on the octadecyl adsorbent. These simple methods can be very useful for rapid estimation of components of peppermint leaves or extracts.