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Rice straw represents a significant energy source for ruminant animals, and fibers and lignin contents of rice straw are negatively related to intake potential of forages. For improvement of the digestibility of rice straw, it is necessary to understand the genetic basis of the related traits. In present study, mapping of quantitative trait loci (QTL) for acid detergent fiber (ADF), neutral detergent fiber (NDF), and acid detergent lignin (ADL) was carried out using a doubled haploid (DH) population derived from a cross between indica variety Zai-Ye-Qing 8 (ZYQ8) and japonica variety Jing-Xi 17 (JX17). The results indicated that all three parameters were continuously distributed among the DH lines, but many DH lines showed transgressive segregation for all the three traits. A total of three main-effect QTLs were identified for ADF and ADL, two of which, qADF-9 and qADL-9, shared the same region on chromosome 9. These two main-effect QTLs explained more than 20% of the total phenotypic variations, whereas the other QTL, qADF-5, explained 12.8% of the total phenotypic variation for ADF. In addition, another two epistatic QTLs, qADF-2 and qADF-3 could explain 17.6% of the total variations. Thus, we concluded that both main-effects and epistatic QTLs were important in controlling the genetic basis of ADF.

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Journal of Thermal Analysis and Calorimetry
Authors:
Yimin Jin
,
J. Bonilla
,
Ye-Gang Lin
,
J. Morgan
,
Linda McCracken
, and
J. Carnahan

Two poly(butylene terephthalate)/polycarbonate (PBT/PC) blends with different formulations were analyzed by modulated DSC (MDSC) and conventional DSC to determine differences in crystallization behavior. A significant difference (30°C in cold crystallization temperature) between the two samples was detectable by MDSC while no significant difference was seen by conventional DSC. That indicatesthe total heat flow from MDSC is not always equivalent to the heat flow from conventional DSC as we have assumed or seen before. The reason has not been fully understood, but may be related to unusual nucleation and crystallization induced by modulation. Alternative conventional DSC methods were developed and compared to the MDSC results.

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The study investigated the effects of environmental factors (salinity, pH, ions and activation media) on sperm motility (activation rate, duration of quick movement, and lifespan) and fertilization rate of Phascolosoma esculenta. The results showed that spermatozoa in the coelom and nephridium are able to move quickly. The optimal salinity was 14.64 to 43.35 and the optimal pH was 6.46 to 9.53 for sperm activation and motility, whereas the ranges for fertilization were narrower (18.56 to 30.3 for salinity and 6.46 to 8.61 for pH). Of the ions studied, Na+ was indispensable for sperm motility and fertilization, and Ca2+ and Mg2+ were necessary for fertilization. P. esculenta sperm could not fertilize eggs and have short lifespans in 200 to 600 mmol/L NaCl and KCl solutions. Furthermore, they could not be activated or move in 200 to 600 mmol/L CaCl2, MgSO4, and sucrose solutions.

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Abstract  

The glass formation and devitrification of GaF3-based glasses were studied by differential scanning calorimetry. A comparison of various simple quantitative methods to assess the level of stability of multicomponent fluoride glass systems is presented. Most of these methods are based on critical temperatures. In this paper a new parameter k b(T) is added to the stability criteria. The stability of several GaF3-based glasses were experimentally evaluated and correlated with the activation energies of crystallization via this new kinetic criterion and compared with those evaluated by other criteria.

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Summary  

The organometallic precursor fac-[99mTc(CO)3(H2O)3]+ was reacted with N-ethoxy, N-ethyl dithiocarbamate (NOET) in phosphate buffered saline (pH 7.4) at room temperature for 30 minutes to produce the 99mTc(CO)3-NOET complex. The radiochemical purity (RCP) of the product was over 90% as measured by thin layer chromatography (TLC). No decomposition of the complex at room temperature (RT) was observed over a period of 6 hours. Its partition coefficient indicated that it was a lipophilic complex. The biodistribution comparison in mice of the 99mTc(CO)3-NOET complex and the 99mTcN-NOET complex showed that the former had a lower heart and brain uptake as compared to that of the latter, suggesting the incorporation of the [99mTc(CO)3]+ core into the NOET ligand does not improve the biological features as a myocardial imaging agent.

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Abstract  

Research on extracted 90Y with di(2-ethylhexyl) orthophosphoric acid (P204) in lipiodol for liver cancer was made to evaluate the stability of extracted 90Y with P204 in lipiodol (90Y-P204-lipiodol) in serum of newly-born cattle and human’s blood. At first, P204 (extractant) was dissolved in lipiodol (organic phase). Secondly, 90Y was extracted to organic phase after adding 90Y solution into test tube with P204 and lipiodol in it. The extracting efficiency with 0.01 mol/l P204 could reach 99.4%. The stability of 90Y-P204-lipiodol has been experimented in physiological saline solution as preparation for further stability experiment. The result indicated that the extracted 90Y lost 0.02%–0.36% in physiological saline solution. The results of further stability experiment showed that loss efficiencies of extracted 90Y after adding newly-born cattle serum 1 hour, 1 day, 3 and 7 days are 3.38%, 3.12%, 4.29% and 6.62%, respectively, and loss efficiencies of extracted 90Y after adding human’s blood 1 hour, 1 day, 3 and 7 days are 2.55%, 5.91%, 7.88% and 5.63%, respectively. Our data also indicated that 90Y is the most possible radioisotope for being extracted with P204 in lipiodol to treat hepatocellular carcinoma, particularly in cases of unresectable liver tumors, since 90Y is available from several commercial sources in clinical quality. We conclude that the stability of 90Y-P204-lipiodol tested with newly-born cattle serum and human’s blood attained great results. 90Y-P204-lipiodol is a kind of potential and exciting pharmaceutical in inerventional therapy for liver cancer and we can carry on the further animal test and clinical trial.

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Abstract  

Hydrogen gas (H2) was produced by gamma-irradiation of pure water in the presence of various TiO2 catalysts. Most catalysts used in this work largely enhanced the radiolytic decomposition of water and subsequent production of H2. The different activity of catalysts was characterized by X-ray diffraction (XRD) patterns and by electron paramagnetic resonance (EPR) spectra. The addition of methanol as a sacrificing agent further increased the H2 production by scavenging hydroxyl radicals and the scavenging activity was directly identified using an EPR/spin-trapping technique. Among the catalysts studied, nanosized TiO2 (nTiO2) showed an excellent activity in the production of H2. Furthermore, the addition of EDTA instead of methanol largely increased the H2 production. This is quite promising since waste compounds such as EDTA can be removed with concomitant H2 production

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Journal of Thermal Analysis and Calorimetry
Authors:
J. Azizi
,
D. Dollimore
,
P. Dollimore
,
G. Heal
,
P. Manley
,
W. Kneller
, and
W. Jin Yong

Abstract  

In this study a relationship between the surface and textural properties of carbon blacks and the gasification process induced thermally in air is noted. A temperature jump method was used to follow the gasification in air and to establish the relevant Arrhenius parameters. This can be associated with the activity of ‘basal’ plane carbon atoms as well as ‘edge’ carbon atoms at the surface. This is based on a model of carbon black structure consisting of the irregular packing of small graphite carbon structures. The carbon black surface was measured using a single measurement of adsorption based essentially on the BET volumetric method. The carbon black surface had a rate of oxidation per unit are which clearly indicated that the lower area carbon blacks had a predominately active area of ‘edge’ atoms at the surface while the surface of the higher area carbon blacks had a predominate amount of ‘basal’ plane graphite carbon atoms.

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Abstract  

The behavior of153Sm-EDTMP in vitro and vivo is analyzed by the size exclusion HPLC. The experimental results show that EDTMP amounts have an obvious effect on the stability in vitro and uptake of153Sm-EDTMP in the liver. HPLC analysis of urine sample indicates that153Sm-EDTMP es excreted in the original form. The behavior in vivo of153Sm-EDTMP containing 4 μg is similar to that of153Sm-EDTMP containing 50 μg EDTMP at 1 h post-injection.

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Abstract  

DTPA-Octreotide(Pentetreotide), a somatostatin analogue which can bind specifically and with high affinity to somatostatin receptor in vitro and vivo, labeled with99mTc by tin reduction in acetate buffer, has been characterized by Reverse-phase High performance Liquid Chromatography. The effect of different solvents, mobile phase pH, linear gradient and the injected volume on the separation efficiency was evaluated. The results show that the separation efficiency is best using μBondapak-C18 (300×3.9 mm2), linear gradient of 40% to 80% methanol (1.0 ml/min) in 0.05M acetate buffer (pH 5.5) over a 30 min period and maintaining for another 10 min. The labeled product is a mixture which mainly consists of five components (a, b, c, d, e) successfully proved by HPLC. Paper chromatography is also evaluated in this paper. It may be used to determine the radiochemical purity of the labeling product, but is not a good choice for the verification each components.

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