Authors:J. Kumar, A. Kumar, M. Kumar, S.K. Singh and L. Singh
In order to study the inheritance pattern of morpho-physiological traits in bread wheat, a 10×10 diallel cross, excluding reciprocals was made and grown in a randomized complete block design (RCBD) with three replications. Observations were recorded on Days to 75% flowering (DF), Days to maturity (DM), Duration of reproductive phase (DRP), Plant height (cm) (PH), Effective tiller/plant (TLS), No. of spikelets per spike (SLS), No. of grains per spike (GS), Grain weight per spike (g) (GW), Spike length (cm) (SL), Biological yield per plant (g) (BY), Harvest index (%) (HI), 1000-Grain weight (g) (TGW), Spike density (SD), Canopy temperature depression (°C) (CTD), Chlorophyll intensity (%) (CI), Chlorophyll fluorescence (Fv/Fm) (CF), Protein content (%) (PC), Grain yield per plant (g) (GY). Highly significant differences were observed among the genotypes for all traits. The resulted 45 F1s and their F2s used for study the nature of gene for grain yield and its contributing traits in bread wheat. The result indicated that considerable gene action and average degree of dominance respond to achieving significant result for grain yield and its component traits. In both the generations F1s and F2s, grain yield per plant (g) was governed by non-additive gene action based on combining ability analysis, (σ2 g/σ2 s)0.5 [GCA and SCA variance ratio] and (H1/D)0.5 [Degree of dominance] were exhibited over dominance type average degree of dominance for grain yield and its component traits in both generations. Genetic analyses of the traits confirm the involvement of both additive and non-additive gene effects in governing the inheritance.
Authors:Avinash Kumar, Deepak Kumar, K. Rao, J. Kumar, B. Singh, A. Sharma and P. Ravi
Prediction of downwind tritium air concentrations in the environment around Narora Atomic Power Station was studied on the
basis of Gaussian plume dispersion model. The tritium air concentrations by field measurement [measured tritium air concentrations
in the areas adjacent to NAPS] were compared with the theoretically calculated values (predicted) to validate the model. This
approach will be useful in evaluating environmental radiological impacts due to standard pressurised heavy water reactors.
Authors:Y. Gautam, S. Sharma, A. Sharma, V. Kumar, J. Kumar, A. Kumar, A. Hegde and P. Sarkar
Guidelines for the assessment of internal doses from monitoring suggest default measurement of uncertainties (i.e. lognormal
scattering factor, SF) to be used for different types of monitoring data. In this paper, SF values have been evaluated for
internal contamination due to 60Co in two cases using whole body counting data. SF values of 1.04 and 1.03 were obtained for case I and II, respectively while
SF value of 1.03 was obtained using bioassay data for case I. SF evaluated is in good agreement with the default values given
by IDEAS guidelines. The present study also presents the follow up study of a case I of 60Co internal contamination using whole body counting and bioassay analysis. The effect of medical intervention applied on the
subject is studied. Medical intervention of d-Penicillamine (250 mg × 4 daily) was orally administered from 13th day of initial exposure for about a fortnight, which showed
reduction of activity present by 33.4% through urine.
The average mealy bug (Phenacoccus solenopsis Tinsley) population was maximum (2.69, 2.40 and 1.73 adults/plant) on Parthenium hysterophorus at wasteland, in or near field and weeds present near stacks at Muktsar. At Ferozepur, the mean mealy bug population was 1.10 adults/plant on cotton. On the weeds, near or in the field, the maximum population (5.94 adult/plant) was recorded on P. hysterophorus followed by Digeria arvensis (3.74 adults/plant). The population was 2.69, 2.66 and 2.86 adults/plant on Sida acuta, Abutilon theophrasti and Achryanthus aspera, respectively. On wasteland weeds, similar trend was observed, i.e. maximum population was on P. hysterophorus (7.31 adults/plant), while minimum (3.00 adults/plant) on S. acuta, during 2008. Similar trend was followed in 2009 in Muktsar and Ferozepur but the population was lower than the previous year. Among the weeds, P. hysterophorus was the most preferred host for multiplication. During carry-over studies all the stages and ovisac was maximum, followed by detached leaves in Muktsar and in Ferozepur again on P. hysterophorus. In Ludhiana, the maximum population was observed on Hibiscus sp. followed by ratoon cotton crop.
is an important medicinal plant widely used in the Unani system of medicine. A simple, precise and convenient HPTLC method has been developed for analysis of chrysophanol, an antioxidant and a major constituent of the rhizomes of
(Polygonacea) and pharmaceutical formulations of these. This method uses chrysophanol as internal standard. The stationary phase was silica gel 60F
, and hexane-ethyl acetate, 45 + 5 (
) was used as mobile phase. Detection and quantification were performed densitometrically at
= 366 nm. The method is characterized by high sensitivity and linearity over a wide concentration range. The results obtained were evaluated statistically.
Molecular markers provide novel tools for linkage mapping of QTLs of target traits and can greatly enhance the efficacy of breeding programs to improve mineral (iron and zinc) density in rice. A F2 population derived from the cross between high-yielding (PAU201) and iron-rich (Palman 579) indica rice varieties displayed large variation for various physio-morphological traits including grain yield per plant and iron and zinc contents. Transgressive segregation for grain iron and/or zinc contents was noticed in some F2 individuals with one of the F2 plants having exceptionally higher iron (475.4 μg/g) as well as zinc (157.4 μg/g) contents. Grain iron content showed significant positive correlation (r = 0.523) with grain zinc content indicating the feasibility of improving iron and zinc levels simultaneously in rice grain. Two parental rice varieties displayed polymorphism at 76 of the 100 SSR loci, which were used to map the QTLs associated with mineral content in grains. Composite interval mapping (CIM) analysis by Win QTL cartographer 2.5 revealed a total of eleven QTLs for mineral content (eight for Fe and three for Zn) in rice grains on chromosomes 2, 3, 7, 10 and 12.
In  Kohli and Vashistha gave an analogue of probabilistic version of Pant‘s Theorem (, Theorem 1). We note that mappings defined in Examples 3.6 to 3.8 of  are not self maps as claimed in the Definitions 3.1 and 3.2. In this context, we provide some relevant examples to complete the interesting results.
Bioassay technique is used for the estimation of actinides present in the body based on their excretion rate through body
fluids. For occupational radiation workers urine assay is the preferred method for monitoring of chronic internal exposure.
Determination of low concentrations of actinides such as plutonium, americium and uranium at low level of mBq in urine by
alpha spectrometry requires pre-concentration of large volumes of urine. This article deals with standardization of analytical
method for the determination of 241Am isotope in urine samples using Extraction Chromatography (EC) and 243Am tracer for radiochemical recovery. The method involves oxidation of urine followed by co-precipitation of americium along
with calcium phosphate. This precipitate after treatment is further subjected to calcium oxalate co-precipitation. Separation
of Am was carried out by EC column prepared by PC88-A (2-ethyl hexyl phosphonic acid 2-ethyl hexyl monoester) adsorbed on
microporous resin XAD-7 (PC88A-XAD7). Am-fraction was electro-deposited and activity estimated using tracer recovery by alpha
spectrometer. Ten routine urine samples of radiation workers were analyzed and consistent radiochemical recovery was obtained
in the range 44–60% with a mean and standard deviation of 51 and 4.7% respectively.
Authors:Saneesh Kumar, Patrick J. Bouic and Bernd Rosenkranz
A simple, efficient, and stable high-performance liquid chromatography (HPLC) separation method for a combination of rifampicin (RIF), its major metabolite 25-O-desacetyl rifampicin (25ODESRIF), and neostigmine (NEO) was developed and validated. The drugs individually, and in combination, were analyzed using a Waters Alliance 2695 HPLC coupled with 2996 photodiode array detector (PDA). Successful separation of combined drugs was achieved by gradient elution on a reverse-phase C-18 Phenomenex Luna column, using a mobile phase consisting of water and methanol at detection wavelength of 254 nm. The HPLC retention times were consistent at ±7.70 min, ±8.25 min, and ±10.70 min for RIF, 25ODESRIF, and NEO, respectively. The regression data for the calibration plots exhibited linear relationship (R2 = 0.995) in the range of 0–200 μM for both RIF and 25ODESRIF, and the lower limit of detection (LLOD) and lower limit of quantification (LLOQ) were calculated at 5.86 μM and 17.75 μM for RIF and 7.78 μM and 23.57 μM for 25ODESRIF, respectively. The method was evaluated using in vitro human liver microsomes (HLMs) assays, and linearity was established for the 15, 30, 45, and 60 min incubations (R2 = 0.99). The formation of 25ODESRIF was characterized by hyperbolic kinetics (Km 48.23 μM, Vmax 1.233 pmol/min/mg protein, and CLint 0.026 μl/min/mg protein). The method was applied in HLM assays to understand the herb–drug interaction (HDI) potential of Althaea officinalis, a popular African herb consumed by tuberculosis (TB) patients, with RIF. None of the extracts of A. officinalis inhibited the esterase-mediated metabolism pathway of RIF, compared to the positive control nelfinavir (IC50 = 9.59 μM). The method provides a tool for quantifying RIF and 25ODESRIF in in vitro drug metabolism assays as well as investigating herb– and drug–drug interactions (DDIs).
Authors:Manoj Kumar, J. Udhayakumar and Ashutosh Dash
This paper describes a novel method for the preparation of 147Pm sources for beta backscatter coating thickness gauge. The process relied on the creation of a porous reactive surface in
a precisely selected region of a long cylindrical aluminum substrate by anodization at a current density of 15 mA/cm2 at 15 °C in 3 M H2SO4 for 2 h. The anodized region was impregnated with 37 MBq (1 mCi) of 147Pm activity by keeping in contact with the radioactive solution at pH 6.0 for 24 h. The active region was subsequently coated
with a thin layer of polymer to prevent the dispersion of activity in use. The sources were submitted to quality control tests
such as non-leachability, uniform distribution of activity and surface contamination.