Authors:G. Galiba, I. Kerepesi, J. W. Snape and A. Vágújfalvi
The mobilization of carbohydrates, especially sucrose, is considered very important during both the cold acclimation process and water stress, while sugars also promote floral transition and cold hardiness. Chinese Spring (CS) 5AL and 5DL deletion lines were studied for the physical assignment of the gene(s) regulating stress-induced sugar accumulation. To separate the effect of cold from that of water deprivation, the seedlings were raised in hydroponics, and apart from the cold, the effect of PEG-induced water stress was also evaluated in a time course experiment. The genes affecting stress-induced carbohydrate accumulation were assigned to the same chromosomal bins, which contain the vernalization genes Vrn-A1and Vrn-D1, on the long arms of chromosomes 5A and 5D, respectively. Sugar accumulation was found to be controlled by Vrngenes in an epistatic manner at least at the beginning of the cold treatment. In the case of cold treatment, Vrn-A1proved to be more effective than Vrn-D1, while in the case of osmotic stress the gene assigned to the long arm of chromosome 5D seemed to be more effective at regulating sugar accumulation than its counterpart on 5A.
Authors:V. Oslovičová, J.R. Simmonds, J.W. Snape, Z. Gálová, Z. Balážová and I. Matušíková
In this study we evaluate the genetic diversity of a selection of wheat accessions characteristically grown and adapted to mid-European environments, using various molecular marker systems. Thirty-three simple sequence repeat (SSR) markers were used alongside genic markers for known dwarfing genes, flowering time genes, and grain hardness genes, namely Rht-B1, Rht-D1, Ppd-D1, Vrn-A1, Vrn-B1, Vrn-D1 and Pinb-D1. In addition, variation was scored for the high-molecular-weight glutenin storage proteins, responsible for dough technological quality. A dendrogram was constructed using the UPGMA algorithm, based on the molecular data and the country of origin, giving an overview of their genetic similarity and relationships. The potential for the use of some agronomic traits in breeding, by providing a basis for multi-trait genetic selection in wheat breeding programs is discussed. Estimating the breeding values of crops using multiple genetic markers might help in breeding for varieties with good technological quality for growing under desired climatic conditions.