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  • Author or Editor: Jyotsana Dwivedi x
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Oxalis corniculata is an important medicinal plant, but chromatographic analysis for the simultaneous quantification of different phenolic compounds has not been performed till date in this plant species. A high-performance thin-layer chromatography (HPTLC) method for the simultaneous quantitative determination of caffeic acid, vanillic acid, and syringic acid in O. corniculata L. methanolic fraction was developed for the first time. In HPTLC, for achieving good separation, the mobile phase of toluene‒ethyl acetate‒formic acid (7:3:1, v/v) was used. The densitometric determination was carried out at 300 nm in reflection/absorption mode. The calibration curves were linear in the range of 100–700 ng per spot for caffeic acid, vanillic acid, and syringic acid. HPTLC analysis has indicated the presence of optimum amount of caffeic acid (0.025 ± 0.0002), vanillic acid (0.018 ± 0.0005), and syringic acid (0.04 ± 0.0006) in the methanolic fractions. The optimized method was successfully applied for the analysis of three major phenolics in O. corniculata L. The proposed method is simple, precise, specific, and accurate. The quantification of these phenolic compounds has not yet been reported in this species which may be utilized for the proper standardization of the drug.

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A high-performance thin-layer chromatography (HPTLC) method for the simultaneous quantitative determination of ursolic acid and β-sitosterol in the methanolic fraction of Paederia foetida L. leaves was developed for the first time. For achieving good separation, a mobile phase of toluene‒ethyl acetate‒formic acid (8:2:0.1, v/v) was used. The densitometric determination was carried out at 550 and 522 nm in reflection/absorption mode for ursolic acid and β-sitosterol. The calibration curves were linear in the range of 100-600 ng per spot for ursolic acid and β-sitosterol. During the analysis, the methanolic fraction of P. foetida L. leaves showed the presence of ursolic acid (0.12 ± 0.05%) and β-sitosterol (0.08 ± 0.12%). The proposed method is simple, precise, specific, accurate, less time-consuming, and cost-effective. The statistical analysis of the data obtained proves that the method is reproducible and selective and can be used for the routine analysis of the reported phenolic compounds in crude drug and extracts. The simultaneous quantification of these compounds has not yet been reported in P. foetida L. leaves which may be utilized for the proper standardization of the plant.

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A high-performance thin-layer chromatography (HPTLC) method for the simultaneous quantitative determination and validation of ursolic acid, β-sitosterol, lupeol, and quercetin in the methanolic fraction of Ichnocarpus frutescens L. was developed for the first time. For achieving good separation, a mobile phase of toluene‒ethyl acetate‒formic acid (8:2:0.1, v/v) was used. Densitometric determination was carried out at 500 nm for ursolic acid, 550 nm for β-sitosterol, 650 nm for lupeol, and 310 nm for quercetin in reflection–absorption mode, and the calibration curves were linear in the range of 100–600 ng per spot. During the analysis, the methanolic fraction of I. frutescens L. showed the presence of ursolic acid (0.34%), β-sitosterol (0.27%), lupeol (0.27%), and quercetin (0.26%). The proposed method is simple, precise, specific, and accurate. The obtained data can be used for routine analysis of reported biomarkers in crude drug and extracts. The simultaneous quantification and method validation of these biomarkers have not yet been reported in I. frutescens L., which may be utilized for the proper standardization of the plant.

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