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The purpose of the present note is to give a number of characterizations of theR 1-axiom and to show that theR 1-axiom is equivalent to the weakly Hausdorff axiom introduced byB. Banaschewski andJ. M. Maranda [2]. In anR 1-space it is shown that the locally compactness property is also open hereditary and that the closure of an almost compact set is the union of the closures of its points. A necessary and sufficient condition is obtained under which a locally compact set dense in anR 1-space is open. Finally a variant of a well-known theorem regarding two continuous functions of a topological space into aT 2-space is formulated forR 1-spaces.

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In a topological spaceX, a T2-distinct pointx means that for anyyX xy, there exist disjoint open neighbourhoods ofx andy. Similarly, T0-distinct points and T1distinct points are defined. In a Ti-distinct point-setA, we assume that eachxA is a Ti-distinct point (i=0, 1, 2). In the present paper some implications of these notions which ‘localize’ the Ti-separation axioms (i=0, 1, 2) requirement, are studied. Suitable variants of regularity and normality in terms of T2-distinct points are shown hold in a paracompact space (without the assumption of any separation axioms). Later T0-distinct points are used to give two characterizations of the RD-axiom.1 In the end, some simple results are presented including a condition under which an almost compact set is closed and a result regarding two continuous functions from a topological space into a Hausdorff space is sharpened. A result which relates a limit pointv to an ω-limit point is stated.

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Acta Veterinaria Hungarica
Authors: Nadja Fodor, S. K. Dube, I. Fodor, E. Horváth, Edith Nagy, V. N. Vakharia, and Altancsimeg Rencendorsh

Direct DNA inoculations were used to determine the efficacy of gene immunisation of chickens to elicit protective immune responses against infectious bursal disease virus (IBDV). Thevp2 gene of IBDV strains GP40 and D78, and thevp2-vp4-vp3 encoding segment of strain D78 were cloned in an expression vector which consisted of human cytomegalovirus (HCMV) immediate early enhancer and promoter, adenovirus tripartite leader sequences and SV40 polyadenylation signal. For purification of vaccine-quality plasmid DNA fromE. coli, an effective method was developed. Chickens were vaccinated by inoculation of DNA by two routes (intramuscular and intraperitoneal). Two weeks later, chickens were boosted with DNA, and at 2 weeks post-boost, they were challenged with virulent IBDV strain. Low to undetectable levels of IBDV-specific antibodies and no protection were observed with DNA encoding VP2. However, plasmids encoding VP2-VP4-VP3 induced IBDV-specific antibodies and protection in the chickens. DNA immunisation opens a new approach to the development of gene vaccines for chickens against infectious diseases.

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