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  • Author or Editor: K. Michalik x
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Abstract  

A differential scanning calorimetry study of the thermal behavior of nevirapine and azidothymidine in water solution was carried out. For nevirapine scan rate dependent and irreversible endothermic peak were found. Thermal degradation of nevirapine as well as NVP – AZT mixture is relatively well described by the model involving only one irreversible step determined by a first-order rate constant. The estimated kinetic constants and activation energies indicate that the degradation process proceeds slower for nevirapine in presence of AZT ligands than without them.

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Abstract  

The thermal denaturation process of bovine and human both fatty acid containing and fatty acid free albumins in aqueous solution was studied by use of differential scanning calorimetry. Human serum albumins were found to be more stable than their bovine counterparts. Fatty acid free albumins were characterized as generally less stable, more susceptible to aggregation, their unfolding endothermic transition was less cooperative and with the smaller degree of reversibility. Deconvolution analysis with using a non-two-state model with two component transitions showed essential differences in the thermodynamic parameters between all studied albumins, particularly regarding the high-temperature component transition.

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Abstract  

Differential scanning calorimetry (DSC) has been applied for studies of blood serum from patients sick with chronic obstructive pulmonary disease (COPD). The denaturation of serum proceeds as endothermic process over the temperature range 45–85 °C. Distinct changes in the shape of DSC curves have been observed for serum from patients with severe stage of COPD (treated with inhaled corticosteroids) relative to serum from healthy individuals. The first moment of the thermal transition with respect to the temperature axis shifts from the normal value of 63.9 ± 0.3 to 65.3 ± 0.7 °C and to 67.6 ± 1.6 °C for early and advanced stages of disease, respectively. The results of our studies suggest age dependence of blood serum denaturation transition.

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