A scaled-up SRCCC apparatus equipped with a 40-L column was constructed on the basis of separations of crude broccoli seed extract and crude radish seed extract using a conventional SRCCC instrument. Scaled-up separation of 500 g of crude broccoli seed extract with n-butanol-acetonitrile-10% (NH4)2SO4 aqueous solution 1:0.5:2 (υ/υ) as solvent system yielded 61.5 g glucoraphanin product of purity 91.2%. Separation of 500 g crude radish seed extract with the same solvents in the ratio 0.5:1:2 (υ/υ) afforded 26.7 g glucoraphenin product of purity 94.5%. Recovery of glucoraphanin and glucoraphenin from the crude extracts was 98.3 and 98.9%, respectively. The results demonstrated that this SRCCC technology is useful for separation of glucosinolates from plant extracts.
The detection of characteristic gamma rays emitted promptly by elements after capture of neutrons is used as a means of radioanalytic analysis. Here it is shown that the method can be used for the measurement of the nitrogen (and therefore protein) content of small animals, those with mass around 3 kg.
Authors:K. Masumoto, K. Iijima, A. Toyoda, and Q. Wang
The spatial distribution of neutrons was measured at the muon science laboratory of KEK by the activation detector method
using an imaging plate for the radioactivity measurement. It was confirmed that this method is highly sensitive to detect
the average neutron dose of 10 µSv/h. The distribution of thermal and epithermal neutrons was also measured in the experimental
room. The cadmium ratio inside the experimental room is one except for the neutron leakage point. The spatial distribution
of neutrons inside the concrete shield of KENS was measured by the same method. Aluminum and gold foils were used for the
measurement of fast and thermal neutrons, respectively. Two dimensional change of the reaction rate of the 27Al(n,α)24Na reaction shows a good agreement with the results calculated by the Monte Carlo simulation using MARS14 code. Thermal and
epithermal neutron flux ratio on the beam axis was measured by the cadmium ratio method. The flux ratios were about 30 and
almost constant for every slot except for the surface of the shield, because the cadmium ratio is 2. This method was very
useful to measure the activity of many pieces of detector simultaneously without any efficiency and decay correction. Wide
dynamic range and high sensitivity are also the merit of this method.
Authors:Y. Zhang, X. An, X. Li, S. Chen, L. Gao, K. Wang, S. Wang, and Y. Yan
Two new y-type HMW-GSs in
with the mobility order of 1Dy12.2
>1Dy12, were identified by both SDS-PAGE and MALDI-TOF-MS. Molecular cloning and sequencing showed that the genes encoding subunits 1Dy12.1*
had identical nucleotide acid sequences with 1,947 bp encoding a mature protein of 627 residues. Their deduced molecular weights were 67,347.6 Da, satisfactorily corresponding to that of 1Dy12.2
subunit determined by MALDI-TOF-MS (67,015.7 Da), but was significantly smaller than that of the the 1Dy12.1*
subunit (68,577.1 Da). Both subunits showed high similarities to 1Dy10, suggesting that they could have a positive effect on bread-making quality. Interestingly, the expressed protein of the cloned ORF from accessions TD87 and TD130 in
co-migrated with subunit 1Dy12.2
, but moved slightly faster than 1Dy12.1*
on SDS-PAGE. The expressed protein in transgenic tobacco seeds, however, had the same mobility as the 1Dy12.1*
subunit, as confirmed by both SDS-PAGE and Western blotting. Although direct evidence of phosphoprotein could not be obtained by specific staining method, certain types of post-translational modifications (PTMs) of the 1Dy12.1*
subunit could not be excluded. We believe PTMs might be responsible for the molecular weight difference between the subunits 1Dy12.1*
Authors:X. Wang, W. Zimmermann, K. Ng, A. Chakraboty, and J. Keller
This article presents results of the experimental investigation on the adsorption of the water vapor on silica gel. Two independent
experimental methods has been used, viz. the constant-volume-variable-pressure (CVVP) system and variable pressure thermogravimetry
(TG). Results from these two methods are compared with each other. Also the isosteric heat of adsorption of this system has
been determined from the equilibrium data. The silica gels investigated here are Fuji Davison type 'A' and type 'RD'. Adsorption
isotherm of water vapor have been measured under a variety of conditions all referring to chiller operation cycles, i.e. temperatures
from 303 to 358 K and pressures from 500 to 7000 Pa. The data collected from the two independent experiments compare very
favorably with each other and their trends are consistent with those of the adsorption chiller manufacturer. This lends significant
weight to our experimental data on silica gel+water systems as being valuable to the adsorption chiller manufacturers and
the scientific community.
Authors:Jinn-Shing Lee, C.-K. Hsu, and Chin-Wang Huang
The effects of Al2O3 and SiO2 additives on the crystallization of calcium phosphate glasses were studied. When the Al2O3 content was higher than 7 mol%, surface devitrification occurred in the glasses. However, for glasses with Al2O3 contents higher than 10 mol%, bulk devitrification predominanted. For the glasses with SiO2, a surface devitrification mechanism predominanted. Non-isothermal DTA techniques were applied in order to establish the
devitrification mechanism, and the kinetic parameters of crystal growth were obtained. The parameter m depends on the mechanism
and morphology of devitrification of calcium phosphate, glass containing SiO2 as additive, the values of m being lower than 1.2. These results indicate that the devitrification is controlled by the reaction
at the glass-crystal interface, or occurs from surface nuclei.
A total reflection X-ray fluorescence (TXRF) analyzer with a special structure is described. Its short X-ray path (about 15 cm) resulted in a high sensitivity, low power consumption and small volume. The structure with double total reflection path is suitable for easy change of exciting source to cover a large element range. The minimum detection limit (MDL) of 6 pg for Co under Cu exciting source and 22 pg for Sr under Mo tube. Some significant works were done, such as the detemination of sulfur content in fuel oils, the non-destructive analysis of an ancient bronze utensil and the regular analysis of tap water.
Authors:W. Jia, G. Ehrhardt, K. Zinn, N. Wang, and A. Ketring
Polypeptide microspheres containing polycysteine crosslinked with polylysine were prepared and radiolabeled with186Re and188Re. High labeling yields with the microspheres with both186Re and188Re (97%) were obtained, and above 99% retention of radiolabels in water in 24 hours was obtained. Rhenum-186 labeled polycysteine and polylysine microspheres (11 ratio, 20 m as mean diameter) were injected intra-articularly into the rear stifes (knee joints) of normal New Zealand white rabbits. About 87% of injected dose was retained in rabbit stifles and adjacent tissues in 96 hours after injection, while most of the activity lost from the joints was excreted in the urine. Due to its simplicity of preparation and radiolabeling, versatility, and biodegradability, this type of conjugate system may become the therapeutics of choice for radiation synovectomy.