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  • Author or Editor: L. A. Lapshina x
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When tobacco mosaic virus (TMV) and potato virus X (PVX) pre­pa­ra­tions were mixed with the sap from the halo zone (HZ) tissues surround­ing homologous virus-induced local lesions in Datura stramonium and Gomphrena globosa leaves, respectively, and the mixtures were incubated for 18 h at 37 °C, the virus particles underwent destruction. Under the electron microscope abnormal (swollen and “thin”) virions were observed in the incubated virus preparations negatively stained with phospho­tung­stic acid. Sometimes the TMV particles were “cut” across into fragments. Treatment of the virus preparations with the sap from the healthy leaves or HZ surrounding heterologous virus-induced local lesions may cause certain destructive changes of virus particles but to a far lesser extent than treatment with the sap from the homologous virus-induced HZ. Possible mechanisms of destruction of the virus particles are discussed.

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It is established that the central area of TMV-induced local lesions developed in detached Datura stramonium leaves, along with the completely collapsed cells (types I and II), contains cells (type III) conserving to a certain degree integrity of their structural components. A characteristic of the type III cells was the accumulation of considerable amount of virus and formation of TMV-specific granular and tubular inclusions. The study of lesion development showed that a proportion of the collapsed cells and cells of type III did not essentially change in the period up from 3 to 5 days after infection of the leaves. These data suggest that the disease development in cells of type III does not lead to a hypersensitive response and is very similar to that in the systemically infected cells.

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