The seed multiplication of genetically modified (GM) plants requires a modification of the multiplication process used for conventional seed. The difference compared to conventionally-bred varieties involves the detection of the modified character during variety maintenance, seed multiplication and processing, the need for separate storage, processing and transportation, the extra cleaning required for the transportation, storage and processing equipment, and the extra administration necessary for the documentation and labelling of GM seed lots. All in all this results in the lower exploitation of seed-producing capacity and in additional costs. The appearance of GM plants also has an effect on the breeders and seed multipliers of conventional varieties, however, since the possibility of contamination cannot be excluded. The producers of seed free of GMs (within the tolerance threshold) are forced to employ costly cultivation techniques (temporal and spatial isolation, removal of volunteer plants) and laboratory tests (for GM contamination) if their varieties are to stay on the market and if it is to remain possible to produce GM-free products in the future.
In Hungary, stem rust epidemics caused by Puccinia graminis f. sp. tritici are rare, but due to the severity of infection the stem rust fungus can pose a great hazard to wheat production. As new virulent races can appear, it is important for breeders to know of the genetic background of the stem rust resistance in their cultivars. In this study, 220 winter wheat cultivars registered in Hungary in the past 35 years were investigated using molecular markers to determine the presence or absence and frequency of the two important stem rust resistance genes Sr31 and Sr36. The results indicated that both Sr31 and Sr36 genes are widespread in wheat cultivars registered in Hungary. Sr31 was detected in 24.1% of these wheats, and Sr36 in 15.9%. These genes occurred to a somewhat larger extent in the 156 local cultivars: one-third (32.1%) had the Sr31 and 18.0% the Sr36 gene. Of these, 2 cultivars (1.3%) had both genes (Sr31+ Sr36). Among the 64 foreign cultivars only 3 (4.7%) carried the Sr31 gene. In the foreign group, Sr36 was only detected in the seven Croatian cultivars. Tests also revealed possible false pedigrees for some cultivars. Inoculation tests showed that both genes were still effective. One-sixth (16.7%) of stem rust resistant cultivars did not carry the target genes indicating the possible presence of other efficient Sr genes. Data may help breeders to incorporate effective Sr genes into new cultivars.
The widespread use of digitally-controlled measuring and analytical devices and electronic data collectors, all equipped with microprocessors and linked to computers, has made it possible for on-line data collection to become a routine process. A rational combination of two up-to-date techniques, barcodes and digital balance terminals, linked to an average computer background (Kuti et al., 2003), has proved in practice to satisfy the criteria raised for the up-to-date processing of breeding data at low cost. This system is an example of how it is possible to reduce costs while processing data more rapidly and reliably and allowing human resources to be utilised more flexibly and efficiently. The modules (MvLabel, MvSticker, MvWeighing)of the program package developed in Martonvásár for the handling and analysis of the data from plant breeding and crop production experiments can also be used independently for the identification of experimental field units (spikes, rows, plots) and for the online handling of weight measurements and analytical data. They provide a simple solution for the design and printing of labels (self-adhesive or plastic) containing barcodes. They make it easier to retrieve the data recorded by digital balance terminals and store them on hard discs, while also helping to unify and synchronise the various parts of the system using barcode readers to identify the measurement data.
Central leader and vase form tree models were built using Finite Element Modelling (FEM). Their main characteristics were chosen to be the same. To get comparable results to real values, acceleration versus time curves of the two types of real trees were processed using FFT method to determine their natural frequencies. The natural frequencies measured on real trees and calculated for the models have shown good similarity. The models were virtually exposed to the effect of horizontal forced vibration in the frequency range of 0–20 Hz. Acceleration-frequency curves were calculated and drawn to find the best frequency values for the highest accelerations and also to see their differences in the limb. For the same purpose, the direction of shaking was also changed. It was found that for the central leader limb shape multidirectional shaking would bring uniform detachment, while for the vase form trees, even the unidirectional shakers are appropriate. Real trees were also shaken and their acceleration-frequency curves were compared with the values of the FEMs. The resultant good similarity proves the ability of the models. The acceleration values achieved in the vase form models were much higher than for the central leader type. The acceleration-frequency curve of the shaker unit can be used to find the best frequency for shaking.
Both resistance genes
. The gene
is derived from two different translocations: 1BS/3Ag (‘Amigo’) or 3DS/3Ag (‘Agent’). The use of molecular markers makes selection easier during the breeding process as well as in the selection of the parents. In this study, two markers were used to identify the gene
) and four different markers (J9/1-2
) were available to search for the gene
. The GbF/
marker for gene
worked well, but the SCAR marker SCS265
proved to be easier to use in MAS. SCAR markers SCS1302
proved to be highly reliable and effective for gene
not only in Agent-derived sources but also in ‘Amigo’ derivatives. The STS marker J9/1-2
and the SCAR marker SC-H5
required several modifications and were effective only in ‘Agent’ offsprings.
The storage of wheat data in computers began in the mid-eighties in Martonvásár, and was accompanied by the development of the first simple programs to assist the data management of routine breeding tasks. The great expansion of breeding materials and the demand for new applications have led to an enormous increase in the number of data and have made data processing increasingly more complicated. Data storage facilities and computer programs reflecting an outdated technological level were unable to keep pace with developments. Data storage and applications had to be redesigned on new lines to create a completely new information system amalgamating know-how from breeding and informatics.The paper introduces an extremely important part of this system: pedigree records, which contain the designations of all the genotypes included in traditional field breeding programmes and in the gene bank, together with crossing data, phenotypes and genomic data.An up-to-date, consistent pedigree register is one of the key components in the breeding information system, without which the maintenance and alteration of the names of plant species (wheat, barley, oats, etc.) and linking them to experiments and experimental quality data would be an extremely complex, time-consuming task. It would be even more difficult to keep track of all the genotypes and the increasingly large numbers of related lines from year to year.In addition to describing the rationale behind the system, details will be given on the tools and conditions required for the establishment of the pedigree records, and the internal and external sources available. Finally, some practical examples will be given of how the Martonvásár wheat breeding information system has been applied.
Authors:B. Kőszegi, G. Linc, Lajos Juhász, L. Láng, and M. Molnár-Láng
Acta Agronomica Hungarica, 48(3), pp. 227–236 (2000) OCCURRENCE OF THE 1RS/1BL WHEAT–RYE TRANSLOCATION IN HUNGARIAN WHEAT VARIETIES B. K Ő SZEGI, G. LINC, A. JUHÁSZ, L. LÁNG and M. MOLNÁR-LÁNG AGRICULTURAL RESEARCH INSTITUTE OF THE HUNGARIAN ACADEMY OF SCIENCES, MARTONVÁSÁR, HUNGARY Received: August 15, 2000; accepted: October 15, 2000 The translocation which involves the substitution of the short arm of the 1R rye chromosome for the short arm of the 1B wheat chromosome by means of centric fusion has exercised an enormous influence on the world’s wheat breeding. Since the first mention of this translocation in 1937 the incidence of the 1RS/1BL translocation has been reported in connection with several hundred wheat varieties. Varieties carrying the translocation possess a chromosome segment which includes the resistance genes Sr31 (stem rust, Puccinia graminis), Lr26 (leaf rust, P. recondita), Yr9 (yellow rust, P. striiformis), Pm8 (powdery mildew, Erysiphe graminis) and Gb (aphid, Schizaphis graminum). The present paper investigates the occurrence of the 1RS/1BL translocation in wheat varieties bred in Hungary in recent years. It was found that 35 (53%) of the 66 Hungarian-bred wheat varieties registered in Hungary between 1978 and 1999 carried the 1RS/1BL translocation.
Authors:J. Bányai, É. Láng, Z. Bognár, C. Kuti, T. SpitkÓ, L. Láng, and Z. Bedő
The effect of irrigation water on the yield and on individual yield components was examined for 19 durum wheat varieties by continually recording weather data and carrying out measurements on the moisture content, temperature, electrical conductivity and tension of the soil. Dry (rain-fed) and irrigated treatments were included in the experiment, which was carried out in the framework of the EU FP7-244374 DROPS project.During the rainless spring of 2011 the soil moisture content of the non-irrigated area dropped to 21–22 vol% and the effect of drought stress was still felt at harvest. The quantity of irrigation water applied during the growing season ensured normal conditions for generative development and a significant difference could be detected between the yield components in the two treatments. The thousand-kernel weight of the varieties was identical in the dry and irrigated plots, but in response to irrigation there was an increase in the number of grains per ear and the grain weight, and an improvement in fertilisation, resulting in higher yields.
Authors:G. Gulyás, Z. Bognár, L. Láng, M. Rakszegi, and Z. Bedő
A total of 266 Martonvásár (Mv) wheat (Triticum aestivum L.) accessions, including varieties and advanced lines, were examined using the “Perfect” molecular markers to detect the Rht-B1b (formerly Rht1) and Rht-D1b (formerly Rht2) semi-dwarfing genes. The gene Rht-B1b was detected in a total of 221 (83.5%) accessions. The Rht-D1b allele was found in fewer accessions. Overall 24 genotypes (9%) contained this allele. The analysis of the development date of the genotypes revealed that the introduction of the dwarfing genes into Martonvásár breeding programmes started in the early 1970s, and they were widely utilized from the 1980s. The Rht-B1b allele was the main source for reducing plant height, while the Rht-D1b allele played only a minor role in the Martonvásár breeding programme.Characterizing accessions using various molecular markers allows us to create a database offering relevant marker information about genotypes. Such a database could be very helpful for selection, allowing breeders to include varieties giving positive results in specific breeding programmes.