An investigation of the quantitative content of Cd and Ti in human serum samples by proton nuclear activation (PNA) has been performed and the results are presented. The activation has been induced by a 13.5 MeV proton beam of the AVF cyclotron of the University of Milan, via a (p, n) reaction on the nuclei of the target. For the quantitative determination a known amount of a reference element has been added to the samples and comparison has been made with a standard sample containing also known quantities of the elements studied. Response linearity, reproducibility and possible contaminations have been tested.
Authors:M. Cantone, G. Gambarini, N. Molho, and L. Pirola
A method for Cr determination in biological samples based on proton nuclear activation is presented. The activation was induced by a 13.8 MeV proton beam of the AVF Cyclotron of Milan University via a (p, n) reaction on the nuclei of the target. For the quantitative determination Cd has been chosen as reference element. The method has been applied to Cr determination in human serum samples. The experimental procedure is described and results are presented and discussed.
Authors:M. Cantone, D. De Bartolo, G. Gambarini, A. Giussani, N. Molho, L. Pirola, Ch. Hansen, E. Werner, and P. Roth
An investigation on tellurium metabolism by administration of stable tellurium isotopes124Te and126Te has been performed. Fractional intestinal absorption was determined in rabbits by the double tracer technique. The investigated subjects were given an enriched solution of one tellurium isotope orally and a few minutes later an enriched solution of the other isotope intravenously. The124Te and126Te contents in plasma samples were determined by proton nuclear activation. The methodology described offers a means to study tellurium metabolism in humans without radiation risk.
Authors:M. Cantone, D. De Bartolo, A. Giussani, N. Molho, L. Pirola, G. Gambarini, Ch. Hansen, P. Roth, and E. Werner
The feasibility of ruthenium metabolism studies by stable tracer administration, with a methodology based on proton nuclear activation, is presented. In order to test that the amount of stable tracer administered does not perturb significantly the mechanism investigated, a series of comparative experiments with administration of both radioactive and stable tracers has been performed on animals. As the most critical pathway seems to be the intravenous injection, four male rabbits were given an intravenous injection of radioactive106Ru. Successively, the rabbits were given either a further injection of radioactive106Ru or injection of different quantities of natural Ru. The activity of106Ru and the concentration of natural Ru were measured in plasma samples withdrawn at different time intervals from the injections and the results were compared. Some biokinetic parameters and tissue distribution of Ru in rabbits were determined.