Authors:A. Bravo, L. Ponce, P. Párraga, R. Oliva, and K. Proaño
In this study we analyzed the performance of three wheat varieties in relation to gluten content under high-altitude growing conditions in the Andes of Ecuador. A field experiment was conducted at 3058 meters above sea level during 2009 using adapted wheat cultivar Cojitambo, cv. Carnavalero, and cv. Sibambe. Transcript accumulations of High Molecular Weight Glutenin Subunits (HMW-GS) genes were also evaluated during grain development using qRT-PCR. We recorded the expression profile of HMW-GS genes during 41 days and showed a coordinated pattern of induction with significant higher levels at 82–86 days. Transcript accumulation of 1Dx5, 1Dy10, 1Bx7, 1Ax1, and 1By9 genes were analyzed in more details during this period. The assay highlighted the specific contribution of 1Bx7, 1Dy10, and 1Dx5 during gluten formation in Ecuadorian wheat varieties. Under Andean highlands conditions, cv. Carnavalero showed the higher values of total agglomerated protein upon hydration and higher levels of expression of particular HMW-GS genes. The data suggest a correlation between wet gluten content and HMW-GS genes expression. Our study contributes to understand gluten formation in wheat endosperm under high-altitude conditions in the Andes.
Authors:E. Quintero-Ponce, H. López-González, and L. Cervantes-Naranjo
The aim of this work was to determine the contamination of I-131 in milk samples by low background -spectrometry and low background -counting techniques in the vicinity of the Nuclear Center in Salazar, Mexico. The low background -spectrometry was done directly. The determination by low background -counting consisted of a radiochemical separation of I-131 by ion exchange from the milk samples followed by measurement in a low background -counting system. The results indicated that there was no contamination of I-131 in the samples.
Authors:H. López-González, E. Quintero-Ponce, and L. Cervantes-Naranjo
The conditions to measure the gross alpha and gross beta radioactivity in water samples from the surrounding of the Nuclear
Center (Instituto Nacional Investigaciones Nucleares, (ININ), and Collection, Treatment and Storage Center for Low Level Radioactive
Waste (Maquixco) in the State of Mexico were established. The samples were collected from 1987 to 1996. The gross alpha and
gross beta radioactivity in the samples were determined by a gas-flow proportional counter. The results indicated that the
gross alpha and gross beta radioactive contamination in water samples were below the maximum contaminant level (MCL).