Search Results

You are looking at 1 - 10 of 12 items for

  • Author or Editor: L. Puskás x
  • Refine by Access: All Content x
Clear All Modify Search

The paper deals with connections between solar flare activities and light-trap collection of insects. The authors have worked out the catch data of European corn borer (Ostrinia nubilalis Hbn.) adults, as published for the period of 1976–1997 by the Hungarian national light-trap network. The results proved that both the daily and annual catches were significantly modified by the Q-indexes, expressing the different lengths and intensities of the solar flares. On days with high Q-indexes relative to the ones of the average swarming periods, the number of catches are considerably lower. In those years when the Q-index is high, the average individual number and the number of caught moths are lower by 30% as compared to the average number of total cycles (11 years) and the average population density of a given biotope. On the contrary, in years with low Q-indexes an increase as high as 45% can be experienced in the number of individuals collected. Thus, by evaluating the light-trap catches a strong modifying effect of solar flares has to be considered.

Restricted access

The studies aimed to acquire the widest possible information on the annual flight in Hungary of the European corn borer (ECB), Ostrinia nubilalis Hübner (Lepidoptera: Pyralidae). The investigations used biomathematical (Part 1) and graphical (Part 2) evaluation to document changes in the individual population number.The study was conducted in Hungary using ECB moth capture records from the Plant Protection Information System black light trap system (1991–2004). We have drawn conclusions on the appearance of annual flights and the tendency of alterations in flight direction by means of light trap results in four different areas in Hungary. We calculated the flight peak quotients, the individual population numbers of the second flight peak, the distinctions of individual numbers of two flight peaks in this part.As previously published, alterations in flight direction of ECB flights began at different times in Hungary. In the current study, a gradual disappearance of the univoltine ecotype and gradual appearance of the bivoltine ecotype ECB in Hungary is confirmed by the data obtained between 1991–2004. Flight peak quotients and data concerning the second flight peak have confirmed change this process, too: the appearance of a second flight peak in Northwestern Hungary from 1995–1996 (FP = 1.27), the more significant appearance of flights in August in Western Hungary (FP = 1.05) and Northeastern Hungary (FP = 1.45), and a three and four times more individual number of the second flight peak in Southeastern Hungary (FP = 3.44). Flight peak quotients, individual population numbers of the second flight peak, the tendency towards a difference in population number of the two peaks, and size of increase of these values demonstrates the southeastern-northwestern presence of the bivoltine ecotype in Hungary.

Restricted access

The studies aimed to acquire the widest possible information on the annual flight in Hungary of the European corn borer (ECB), Ostrinia nubilalis Hübner (Lepidoptera: Pyralidae). The investigations used biomathematical (Part 1) and graphical (Part 2) assay to document forthcoming changes in the individual population number.The study was conducted in Hungary using ECB moth capture records from the Plant Protection Information System black light trap system (1991–2004). We have drawn conclusions on the appearance of annual flights and the tendency of alterations in flight direction by means of light trap results in four different areas in Hungary. We prepared and estimated the flight phenology diagrams of ECB in this part.Assay of 224 flight phenology diagrams showed the phenomenon of flight alteration too, which characterized the cyclical change of different phenology types. We determined four well-differentiated flight types. The European corn borer (ECB) univoltine ecotype is characterized by a “bell” type and the bivoltine ecotype is characterized by “nail” type. We also ascertained two temporary types. The “plateau” type can be observed in the initial period of flight alteration, and the “valley” type in the latter stages of flight alteration.

Restricted access

The change of the number of turnip moth (Scotia segetum Schiff.) females was examined at 65 different light trap stations between 1957 and 1990. There are two generations of the examined species in Hungary, but the indi­vi­dual number of the first generation is low year by year, so we process­ed only the data of the second generation. Only those generations were examined that had more than 100 samples because of the statistical reliability. Thirty-three light trap stations were suitable for this examination. The joint number of yearly caught male and female individuals and also separately the samples of females were summarised for each observing station. The change of female individual number as a function of total individual number was determined from the coherent pairs of value. The connection can be described by linear function. The proportion of females is 38%. The relative frequency of females was also determined in the function of the individual number. The shown point-mass is similar to the damped vibration known at technical systems. It was established that the female proportion was higher than the average if the individual number is low, but it showed average value when the individual number was high. It can also be established that the female proportion is significantly high in some cases before gradation years.

Restricted access

European corn borer (ECB) flight and ecotype spread examinations were made in Hungary with the help of catching results of 44 agricultural Jermy light traps (2004). We wondered about alteration of flight types spread borderline as a function of global climate change. Catching data were evaluated by simple mathematical proportional numbers. Catching results originating from different points of the country were compared with Walter-Lieth climate diagram (2004) and Péczely’s Hungarian climate districts. Latter was to reveal correlations of flight types and different climatic districts.The previously published flight alteration tendency of ECB (Keszthelyi 2003, 2004b) continued in 2004. Generation quotients proved this process too. Average generation quotient of populations in South-eastern Hungary was 6, and the top of the same rate in this district was 10,84. The earlier observed „one peak flight” type was replaced by „two peaks flight” type in North-western Hungary (average generation quotient of this district: 2,5). The 1RIN (relative individual number per one day) shows regressive tendency from South-eastern Hungary to North-western Hungary (1RIN of 1.district: 6,99; 1RIN of 4.district: 4,69; 1RIN of 10.district: 2,78), but unequivocal conclusions cannot be drawn from these values for places of ecotypes. There is no unambiguous connection between Péczely’s Hungarian climate districts and spread of ECB flight types as proved by the statistical examinations.

Restricted access

We made an examination using the data of higher and lower light-traps of Talcal, Rezi and Kecskemét. We made comparison between the catch and the moon phases.Our results, got from the data of Kecskemét light-trap, prove the proportion of caught samples both of fall webworm ( Hyphantria cunea Drury) and of turnip moth ( Scotia segetum Schiff.) is the most equable at the lower and higher levels at the time of full moon.The proportion of Microlepidoptera individuals (Tarcal), caught by lower and higher light-traps at Tarcal, is the highest at new moon, but it is higher at full moon than at first and last quarter and the proportion of caught species number is also similar.The proportion of Macrolepidoptera individuals (Rezi), caught by lower and higher traps is highest in the last quarter, the lowest in first quarter and at full moon. The proportion of caught species shows similar but more strikingly marked picture.

Restricted access
Acta Biologica Hungarica
Authors:
L. G. Puskás
,
L. Tiszlavicz
,
Zs. Rázga
,
L. L. Torday
,
T. Krenács
, and
J. Gy. Papp

Recent and historical evidence is consistent with the view that atherosclerosis is an infectious disease or microbial toxicosis impacted by genetics and behavior. Because small bacterial-like particles, also known as nanobacteria have been detected in kidney stones, kidney and liver cyst fluids, and can form a calcium apatite coat we posited that this agent is present in calcified human atherosclerotic plaques. Carotid and aortic atherosclerotic plaques and blood samples collected at autopsy were examined for nanobacteria-like structures by light microscopy (hematoxylin-eosin and a calcium-specific von Kossa staining), immuno-gold labeling for transmission electron microscopy (TEM) for specific nanobacterial antigens, and propagation from homogenized, filtered specimens in culture medium. Nanobacterial antigens were identified in situ by immuno-TEM in 9 of 14 plaque specimens, but none of the normal carotid or aortic tissue (5 specimens). Nanobacteria-like particles were propagated from 26 of 42 sclerotic aorta and carotid samples and were confirmed by dot immunoblot, light microscopy and TEM. [3H]L-aspartic acid was incorporated into high molecular weight compounds of demineralized particles. PCR amplification of 16S rDNA sequences from the particles was unsuccessful by traditional protocols. Identification of nanobacteria-like particles at the lesion supports, but does not by itself prove the hypothesis that these agents contribute to the pathogenesis of atherosclerosis, especially vascular calcifications.

Restricted access
Acta Biologica Hungarica
Authors:
Nóra Faragó
,
Ágnes Zvara
,
Z. Varga
,
P. Ferdinandy
, and
L. Puskás

Micro RNAs (miRNA) are an abundant class of small RNAs that regulate the stability and translation of cognate mRNAs. MiRNAs are potential diagnostic markers, moreover, they play an essential role in the development of various heart disesases. In case of limited tissue material, such as, e.g. human biopsies, purification of miRNAs with sufficient yield is critical. Reproducible expression analysis of miRNAs is highly dependent on the quality of the RNA, which is often difficult to achieve from fibrous tissue such as the heart. Several companies developed general purification kits for miRNAs, however, none of them are specialized to fibrotic tissues. Here we describe an optimized miRNA purification protocol that results in high miRNA yield as compared to other methods including trizol-based and column-based protocols. By using our improved protocol, miRNA obtained from heart tissue gave more reproducible results in QRT-PCR analysis and obtained more significant calls (172 vs. 118) during DNA microarray analysis when compared to the commercially available kit. In addition to the heart tissue, the present protocol can be applied to other fibrotic tissues, such as lung or skeletal muscle to isolate high-purity miRNA.

Restricted access

As in the case of other wheat diseases, adult plant resistance (APR) to powdery mildew remains effective longer than monogenic hypersensitive resistance, so the objective was to identify winter wheat genotypes with this type of resistance. Field and greenhouse tests conducted on 41 varieties and breeding lines indicated that 36 were susceptible in the seedling stage, and only five were resistant in all stages of development. It is probable that these latter genotypes contain major resistance genes. The area under the disease progress curve was the same for most of the wheat genotypes as for the APR control variety Massey, but varieties and lines with significantly better resistance were also identified. Among the genotypes in the Martonvásár breeding stock, Mv Táltos and the line Mv07-03 were found to have excellent adult plant resistance.

Restricted access

The aim of the present study was to identify gene expression changes in the rapid cardiac pacing-induced delayed antiarrhythmic protection in the canine, using cDNA microarrays and quantitative real-time PCR (QRT -PCR) techniques. In all dogs under light pentobarbitone anaesthesia, a pacing electrode was introduced into the right ventricle, and then the animals were divided into three groups: (1) sham-operated and sham-paced group (SP, n = 3) (2) ischaemic control group (IC; n = 3); these were without cardiac pacing and subjected only to a 25 min occlusion of the left anterior descending coronary artery (LAD), and (3) paced group (PC, n = 3); these animals were paced at a rate of 220–240 beats min−1 24 h prior to ischaemia. With cDNA chip 23 genes were found with altered expression in response to rapid cardiac pacing and 10 genes in the IC group when compared to SP dogs. These genes encode transcription factors (MEF2); members of signaling pathways (TGFβ2, PDE4D9), hormone related proteins (e.g. vasopressin V1 and V2 receptors). RT-QPCR was used either to confirm the results of the microarray analysis and also to study 46 genes which are already known to have a role in the late phase of PC. By this method 17 genes were up-regulated and 6 genes down-regulated in the IC group; their expression ratios changed either to the opposite or showed no alteration after cardiac pacing. This study would add some new information about those transcriptional changes that are involved in the delayed phase of cardiac protection.

Restricted access