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Journal of Radioanalytical and Nuclear Chemistry
Authors:
R. Bilek
,
R. Hampl
,
Z. Putz
, and
L. Stárka
Abstract
The preparation of reagents, suitable for radioimmunoassay of 17
-hydroxy-4-androsten-3-one /epitestosterone/ in body fluids is described. It includes a simplified synthesis of the immunogen, epitestosterone-3-/O-carboxymethyl/oxime coupled to bovine serumalbumin, its characterization and preparation of the radioligand [125I] iodohistaminyl derivative of epitestosterone. The assay protocol for epitestosterone detection in urine avoiding extraction is described and the reliability criteria of the method are given.
