Authors:A. Lucaciu, M. Frontasyeva, E. Steinnes, Ye. Cheremisina, C. Oprea, T. Progulova, L. Staicu and L. Timofte
This is the first systematic study of air pollution from heavy metals and other trace elements throughout several industrialized
areas and national parks of Eastern Romanian Carpathians using the moss biomonitoring technique. Samples ofHylocomium splendens collected at 120 sites were analyzed by epithermal neutron activation analysis at the pulsed fast reactor IBR-2 at JINR,
Dubna, for a wide range of elements including heavy metals and rare earths (Na, Mg, Al, Cl,K, Ca, Sc, V, Cr, Mn, Fe, Co, Ni
(by (n,p) reaction), Cu, Zn, As, Se, Br, Rb, Sr, Zr, Mo, Ag, Sn, Sb, I, Cs, Ba, La, Ce, Nd, Sm, Eu, Gd, Tb, Yb, Hf, Ta, W,
Au, Th, and U). Copper, lead and cadmium were determined by AAS. Certified Reference Standards of mosses produced for interlaboratory
comparisons in the European moss-survey 1995 were used to ensure the quality of the measurements. To present results in the
form of coloured contour maps the geographical information system GIS-INTEGRO was used. The regional extent of pollution with
specific metals was determined. Results reported are well consistent with those obtained in numerous recent projects carried
out in Germany, the Netherlands, Poland, Bulgaria and other countries.
Authors:Y.-E. Chen, S. Jin, Q.-S. Zhao, Y. Zhang and C.-L. Wang
Lipopolysaccharide and b-1,3-glucan binding protein (LGBP) is a pattern recognition receptor that can recognize and bind LPS and b-1,3-glucan. LGBP has crucial roles in innate immune defense against Gram-negative bacteria and fungi. In this study, LGBP functions in Portunus trituberculatus innate immunity were analyzed. First, the mRNA expression of PtLGBP in hemocytes, hepatopancreas, and muscle toward three typical pathogen-associated molecular patterns (PAMPs) stimulations were examined using real-time PCR. Results show that the overall trend of relative expressions of the LGBP gene in three tissues is consistent, showing up-down trend. In each group, the highest expression of the LGBP gene was at 3 and 12 h post-injection. The LGBP gene is also expressed significantly higher in the hemocytes and hepatopancreas than in the muscle. The highest level of LGBP was in the lipopolysaccharides (LPS) and glucan-injected group, whereas the lowest level was in the PGN-injected group. Furthermore, bacterial agglutination assay with polyclonal antibody specifically for PtLGBP proved that the recombinant PtLGBP (designated as rPtLGBP) could exhibit obvious agglutination activity toward Gram-negative bacteria Escherichia coli, Vibrio parahaemolyticus, and V. alginolyticus; Gram-positive bacteria Bacillus subtilis; and fungi Saccharomyces cerevisiae. LGBP in Portunus trituberculatus possibly served as a multi-functional PRR. In addition, LGBP is not only involved in the immune response against Gram-negative and fungi, as manifested in other invertebrates, but also has a significant role in anti-Gram-positive bacteria infection.
Authors:Jeremy Loenneke, C. Fahs, R. Thiebaud, L. Rossow, T. Abe, Xin Ye, D. Kim and M. Bemben
The purpose of this study was to investigate the potential mechanisms behind the blood flow restriction (BFR) stimulus in the absence of exercise. Nine participants completed a 10 minute time control and then a BFR protocol. The protocol was five, 5-minute bouts of inflation with 3-minutes of deflation between each bout. The pressure was set relative to each individual’s thigh circumference. Significant increases in muscle thickness were observed for both the vastus lateralis (VL) [6%, p = 0.027] and rectus femoris (RF) [22%, p = 0.001] along with a significant decrease in plasma volume [15%, p = 0.001]. Ratings of discomfort during the BFR protocol peaked at 2.7 (light discomfort). There were no significant changes with whole blood lactate, electromyography (EMG), or heart rate (HR), however, there was a trend for a significant increase in HR during the 5th inflation (p = 0.057). In conclusion, this is the first study to demonstrate that the attenuation of both muscle atrophy and declines in strength previously observed with brief applications of BFR may have been mediated through an acute fluid shift induced increase in muscle size. This is supported by our finding that the changes in muscle thickness are maintained even after the cuffs have been removed.
Authors:H. Xie, Z. Lin, Z. Zhang, L. Du, Z. Xin, Y. Ma, X. Ye and X. Chen
The common wheat line, YW243, developed in our research group, was tested for the resistances of barley yellow dwarf virus (BYDV), powdery mildew (Pm) and stripe rust in field, and was analyzed by molecular markers for convenient trace of the resistant genes in breeding. Genomic in situ hybridization (GISH) analysis and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assay further demonstrated that YW243 was a homozygous multiple translocation line of
Triticum aestivum, Thinopyrum intermedium
(T7DS·7DL-7XL & 1BL·1RS). The disease resistance test and marker analysis showed that YW243 carried seven resistance genes to the three diseases, including
to BYDV on 7DL-7XL,
to powdery mildew on 2AL,
Yr2, Yr9, Sr 31
and a new
to stripe rust on 7B, 1BL, 1RS and 2BL. Restriction fragment length polymorphism (RFLP) markers
, sequence tagged site (STS) marker STS
, simple sequence repeat (SSR) markers
, SSR markers
can be used as diagnostic tools to track
Bdv2, Pm4, Yr2, Yr9
, respectively; and two amplified fragment length polymorphism (AFLP) markers
can also be used to select
Authors:Y. Wang, M. Xu, G. Yin, L. Tao, D. Wang and X. Ye
Although significant progress has been made on
-mediated wheat transformation, the current methodologies use immature embryos as recipient tissues, a process which is labor intensive, time consuming and expensive. In this study, we have managed to develop an
-based transformation scheme using explants derived from mature embryos. Based on transient expression of
-glucuronidase (GUS) marker, mature embryo halves prepared from freshly imbibed seeds were generally most susceptible to
-mediated T-DNA transfer. According to the results of callus induction and shoot production, Yumai 66 and Lunxuan 208 showed higher selection and regeneration efficiency than Bobwhite. In line with this finding, fertile T
transgenic plants were most readily obtained for both spring and winter wheat when mature embryo halves were used for co-inoculation by
cells. The presence of the antibiotic selection marker (
, encoding neomycin phosphotransferase II) in the T
plants was revealed by both genomic PCR amplification and the enzyme-linked immunosorbent assay (ELISA). Additional analysis showed that the transgene was stably inherited from the two different generations and segregated normally among the T
progenies. Further development along this line will raise the efficiency of wheat transformation and increase the use of this approach in the molecular breeding of wheat crop.
Authors:A. He, M. Ye, Z. Tang, S. Lu, Y. Gu, X. Fan, L. Zhao and J. Gao
The recombination of hydrogen and oxygen in technical gaseous waste of nuclear power plants in enlarged scale experiment has
been studied on the basis of our previous work.1 The catalyst and its best operating conditions for recombination of hydrogen and oxygen determined in a small scale experiment
were demonstrated and tested. The results show that the data obtained in an enlarged scale experiment agreed well with that
of in a small scale test. The recombination rate of H2 and O2 was higher than 98.3% and 99.98% respectively. After recombination, the residual concentrations of H2 and O2 in waste gas were O2<3 ppm, H2<400 ppm. The Pd-Al2O3 catalyst and operating conditions determined for gaseous waste processing of nuclear power plants were satisfactory.
Authors:S. Xiao, J. Zhang, X. Li, L. Ye, H. Gu and N. Ren
A ternary binuclear complex of dysprosium chloride hexahydrate with m-nitrobenzoic acid and 1,10-phenanthroline, [Dy(m-NBA)3phen]2·4H2O (m-NBA: m-nitrobenzoate; phen: 1,10-phenanthroline) was synthesized. The dissolution enthalpies of [2phen·H2O(s)], [6m-HNBA(s)], [2DyCl3·6H2O(s)], and [Dy(m-NBA)3phen]2·4H2O(s) in the calorimetric solvent (VDMSO:VMeOH = 3:2) were determined by the solution–reaction isoperibol calorimeter at 298.15 K to be
Authors:L. Tian, N. Ren, J. Zhang, H. Liu, S. Sun, H. Ye and K. Wu
The two complexes of [Ln(CA)3bipy]2 (Ln = Tb and Dy; CA = cinnamate; bipy = 2,2′-bipyridine) were prepared and characterized by elemental analysis, infrared
spectra, ultraviolet spectra, thermogravimetry and differential thermogravimetry techniques. The thermal decomposition behaviors
of the two complexes under a static air atmosphere can be discussed by thermogravimetry and differential thermogravimetry
and infrared spectra techniques. The non-isothermal kinetics was investigated by using a double equal-double steps method,
the nonlinear integral isoconversional method and the Starink method. The mechanism functions of the first decomposition step
of the two complexes were determined. The thermodynamic parameters (ΔH≠, ΔG≠ and ΔS≠) and kinetic parameters (activation energy E and the pre-exponential factor A) of the two complexes were also calculated.
Authors:H. Ye, N. Ren, H. Li, J. Zhang, S. Sun and L. Tian
The complex of [Nd(BA)3bipy]2 (BA = benzoic acid; bipy = 2,2′-bipyridine) has been synthesized and characterized by elemental analysis, IR spectra, single
crystal X-ray diffraction, and TG/DTG techniques. The crystal is monoclinic with space group P2(1)/n. The two–eight coordinated Nd3+ ions are linked together by four bridged BA ligands and each Nd3+ ion is further bonded to one chelated bidentate BA ligand and one 2,2′-bipyridine molecule. The thermal decomposition process
of the title complex was discussed by TG/DTG and IR techniques. The non-isothermal kinetics was investigated by using double
equal-double step method. The kinetic equation for the first stage can be expressed as dα/dt = A exp(−E/RT)(1 − α). The thermodynamic parameters (ΔH≠, ΔG≠, and ΔS≠) and kinetic parameters (activation energy E and pre-exponential factor A) were also calculated.
Authors:L. Mosulishvili, Ye. Kirkesali, A. Belokobylsky, A. Khizanishvili, M. Frontasyeva, S. Gundorina and C. Oprea
To evaluate the potentiality of the blue-green algae Spirulina platensis as a matrix for the production of Se-containing pharmaceuticals, the background levels of 31 major, minor and trace elements (Na, Mg, Al, Cl, K, Ca, Sc, V, Cr, Mn, Fe, Co, Ni using (n,p) reaction), As, Br, Zn, Rb, Mo, Ag, Sb, I, Ba, Sm, Tb, Tm, Hf, Ta, W, Au, Hg, Th were determined in Spirulina platensis biomass by means of epithermal neutron activation analysis. The possibility of the purpose-oriented incorporation of Se into Spirulina platensis biomass was demonstrated. The polynomial dependence of the Se accumulation on nutritional medium loading was revealed. The analytical technique used allows to control the amount of toxic elements in algae Spirulina platensis. Based on this study, a conclusion of the possibility to use Spirulina platensisas a matrix for the production of Se-containing pharmaceutical was drawn.