Authors:A. Usherov-Marshak, L. Pershina, and P. Krivenko
This paper presents results of thermochemical and thermokinetic studies of the hydration of portland cement, alkaline cements
with mineral additives and slag alkaline binder. The influence of the modulus of basicity of the binders on the thermochemical
and thermokinetic characteristics of hydration was estimated.
Authors:E. Khlestkina, E. Antonova, L. Pershina, A. Soloviev, E. Badaeva, A. BÖrner, and E. Salina
Anthocyanin accumulation in vegetative organs has a relationship to stress resistance in plants. In wheat, ability to accumulate anthocyanins in the coleoptile is inherited and controlled by the Rc (red coleoptile) genes. The aim of the current study was to find potential sources of ‘strong’ Rc alleles conferring very high levels of anthocyanin production and to study the effect of genetic background on Rc expression. We measured the relative anthocyanin content (OD530) in the coleoptile of different wheat and wheat-alien genetic stocks and accessions to find potential sources of ‘strong’ Rc alleles conferring very high levels of anthocyanin production. The OD530 values varied from 0.514 to 3.311 in genotypes having red coleoptiles. The highest anthocyanin content was detected in coleoptiles of four Triticum dicoccoides accessions originating from Israel and the Russian T. aestivum cultivar ‘Novosibirskaya 67’, suggesting that their Rc alleles can be used to increase anthocyanin content in the coleoptile of wheat cultivars. It is also suggested that rye Rc alleles, such as that of Russian cultivar ‘Selenga’, can be used to increase anthocyanin content in triticale seedlings.
Authors:N.V. Trubacheeva, E.D. Badaeva, T.S. Osadchaya, and L.A. Pershina
Wild barley, Hordeum marinum subsp. gussoneanum (2n = 28) is a valuable source of genes that determine resistance to abiotic stresses. These resistance traits might be transferred to wheat due to the crossability of wild barley with bread wheat. The availability of reliable and rapid methods for the identification of H. marinum subsp. gussoneanum chromatin in a wheat background would facilitate the development of introgression wheat genotypes. For this purpose, we evaluated the applicability of eighty-seven H. vulgare EST markers for studying bread wheat – H. marinum subsp. gussoneanum substitution and addition lines. Of all of the markers studied, forty-three (49%) were amplified in H. marinum ssp. gussoneanum and wheat introgression lines. The identification of wild barley chromosomes using EST markers confirmed the GISH and C-banding data. Thus, it was established that the H. vulgare EST markers can be successfully used to identify the chromosomes of the H. marinum subsp. gussoneanum in introgression lines of wheat.
Authors:E.V. Chumanova, T.T. Efremova, N.V. Trubacheeva, and L.A. Pershina
The effects of alien substitutions of chromosomes on the heading time of ditelosomic (DT) wheat–barley substitution lines in which the chromosome 7HLmar from Hordeum marinum ssp. gussoneanum 4x replaced the chromosomes 7A, 7B and 7D of common wheat were studied. The plants were grown under short and long day illumination in greenhouse and in the field. The lines studied were found to differ in response to the length of the day. Under short day conditions, DT7HLmar(7B) and DT7HLmar(7A) showed an increase in the period before heading. In this case, the substitution effect of chromosome 7B was more significant than the effect of chromosome 7A. Under these conditions, the substitution of chromosome 7D did not have a significant effect on the heading time. Under long day conditions in the greenhouse and under natural conditions of a long day in the Novosibirsk region, substitution lines came into ear earlier than under a short day conditions and did not differ in the heading time. Allele-specific primers established the allelic composition of the genes Vrn-A1, Vrn-B1, Vrn-D1 and Vrn-B3 in ditelosomic lines. It was shown that the two DT7HLmar(7A) and DT7HLmar(7D) lines have the same genotype -VRN-A1b/VRN-B1c/vrn-D1/ vrn-B3 and that the DT7HLmar(7B) line has the genotype -VRN-A1a/VRN-B1c/vrn-D1. The results show that regardless of the genotype for the Vrn genes, the wheat-barley substitution lines react to the change in the photoperiod, especially in the absence of chromosomes 7B and 7A.