Authors:Cibele Zamboni, Laura Oliveira, Luciana Kovacs, and Sabrina Metairon
In this study the neutron activation analysis (NAA) technique was applied to determine Ca and Mg in whole blood from inhabitants
of Brazil for the purpose of establishing concentration ranges indicative of sex and age. The initiative to perform these
measurements is related to the increase in heart disease. According to recent statistics from WHO, the average is one death
due to heart attack in Brazil, every five minutes. The measures were performed considering lifestyle factors (non-smokers,
non-drinkers and no history of toxicological exposure) of Brazilian inhabitants. A healthy group constituted of male (n = 94) and female (n = 84) blood donors, ages between 18 and 70 years and above 50 kg, was selected from the blood banks and hematological laboratories
of Brazil. The influence of sex was also investigated considering several age ranges (18–29, 30–40, 41–50, >50 years). The
results show significant differences when a comparison is made by sex and age and may be useful to identify or prevent clinical
diseases. These results emphasize the need to perform periodic evaluation of Ca and Mg in blood.
Authors:Laura Oliveira, Cibele Zamboni, Edson Pessoa, and Fernanda Borges
In this investigation ions in serum, urine and kidney of Wistar rats (control group) and Wistar with Acute Renal Insufficiency
(ARI) were quantified using instrumental neutron activation analysis. The measurements in serum and urine were performed before,
during and after ischemia-induced ARI. The measurements in kidney were performed for the control and ARI groups. Also, a comparative
analysis between the concentration ratios before, during and after ARI was performed in urine and serum samples for both groups.
The variations results for Cu in serum and I in urine, before and after ischemia-induced ARI, suggest that these elements
must be also investigated in renal dysfunction.
Authors:Cibele Zamboni, Sabrina Metairon, Laura Oliveira, Simone Simons, A. Chudzinski-Tavassi, and Daniella Oliveira
The determination of elemental concentrations for Br, Ca, Cl, Fe, K, Mg, Na, S, Sr, and Zn in blood samples from White New
Zealand rabbits was performed applying the NAA technique. Twenty whole blood samples (12 male and 8 female) collected in research
centers from Brazil (Aggeu Magalhães in Recife and Butantan Institute in São Paulo) were investigated, using the IEA-R1 nuclear
reactor at IPEN/CNEN-SP-Brazil. These data can be used as references to perform biochemistry analyses in veterinary medicine
using small quantities of whole blood (100–400 μL), simplifying the collection and the preparation of biological samples (it
is not necessary to perform the serum separation nor to use specific reactants). Furthermore, the knowledge of the biochemical
values in blood allows us to check the similarities with the blood estimations in human beings, which is an important condition
for selecting laboratory animals. Finally, these data suggest a great similarity of the inorganic tissue profile of rabbits
(White New Zealand) and humans.