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- Author or Editor: Márta Wilhelm x
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Glial elements in the central nervous system of Eisenia fetida were studied at light- and electron microscopic level. Cells were characterized with the aid of toluidine blue, Glial Fibrillary Acidic Protein (GFAP), S100 staining. We identified neurilemmal-, subneurilemmal-, supporting-nutrifying- and myelinsheath forming glial cells. Both neuronal and non-neuronal elements are S100-immunoreactive in the CNS. Among glial cells neurilemmal and subneurilemmal cells are S100-immunopositive. With the antibody against the S100 protein one band is visible at 15 kDa. GFA P-immunopositive supporting-nutrifying glial cells are localized around neurons and they often appear as cells with many vacuoles. GFA P-positive cell bodies of elongated neurilemmal glial cells are also visible. Western blot analysis shows a single 57 kDa GFA P immunoreactive band in the Eisenia sample. At ultrastructural level contacts between neuronal and glial cells are recognizable. Glial cell bodies and their filopodia contain a granular and vesicular system. Close contacts between neuronal cell membranes and glial filopodia create a special environment for material transport. Vesicles budding off glial cell granules move towards the cell membranes, probably emptying their content with kiss and run exocytosis. The secreted compounds in return may help neuronal survival, provide nutrition, and filopodia may also support neuronal terminals.
This study compared the physical fitness of Hungarian undergraduate students in 2011–2012 with the representative sample surveyed in 1997–1998. A total of 123 males and 309 females (mean age 21.19±2.19 yrs) were randomly selected in two Hungarian universities (Pécs, Kaposvár). Anthropometric data (height, weight, skinfolds, body fat percentages) were measured and the subjects performed 9 tests of the Eurofit Fitness Test Battery. The BMI, total body fat and performance in most of the fitness components (balance, agility/speed, flexibility, abdominal muscular strength, aerobic fitness of females) was higher in 1997–1998 (p<0.05) whereas the handgrip strength and performance in Bent Arm Hanging test were significantly better in 2011–2012 (p<0.001). These findings support interventions focusing on increasing regular physical activity among Hungarian youth.
Several studies have described high correlation of salivary and blood lactate level during exercise. Measuring the effectiveness and intensity of training, lactate concentration in blood, and lately in saliva are used.The aim of our study was to evaluate the correlation between the concentration and timing of salivary and blood lactate level in endurance athletes and non-athletes after a maximal treadmill test, and to identify physiological and biochemical factors affecting these lactate levels.Sixteen volunteers (8 athletes and 8 non-athletes) performed maximal intensity (Astrand) treadmill test. Anthropometric characteristics, body composition and physiological parameters (heart rate, RR-variability) were measured in both studied groups. Blood and whole saliva samples were collected before and 1, 4, 8, 12, 15, 20 min after the exercise test. Lactate level changes were monitored in the two groups and two lactate peaks were registered at different timeperiods in athletes. We found significant correlation between several measured parameters (salivary lactate — total body water, salivary lactate — RR-variability, maximal salivary lactate — maximal heart rate during exercise, salivary- and blood lactate −1 min after exercise test). Stronger correlation was noted between salivary lactate and blood lactate in athletes, than in controls.
