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  • Author or Editor: M. Abdel-Azeez x
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Abstract  

Correlation between the in vivo distribution and the chemical formulation of99mTc-PYP complex has been studied. We chose mice to evaluate in vivo biodistribution and gel chromatography column scanning technique for radiochemical analysis. The influence of the pH, Sn(II), pyrophosphate concentration and molar ratios of Sn: PYP on the labelling of pyp with99mTc has been investigated in vitro and in vivo. The reaction between99mTc and Sn-PYP was complete within a few minutes. The stability studies were evaluated against dilution. Induced myocardial infarction was evaluated in rats. The clinical evaluation showed excellent definition of sternum and ribs with little blood background activity with normal subjects. Discrete localization of abnormally high activity was shown in the site of recent infarction of the left ventricular myocardium.

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Abstract  

The distribution of four different commercially available A, B, C, D kits (99mTc-sulfur colloid) for hepatoimaging was compared in mice by organ radioassay and in rabbits for blood clearance. The distribution of kits A and C (single step kits) was assessed in the human by blood clearance, external liver, spleen measurement, and scintillation camera imaging. Kit A reaches a high concentration in liver within 15–20 minutes with relatively high surrounding tissue background, and superior spleen scintiphotos. However, when kit C was used, a high activity concentration in the liver was reached within 10–15 minutes with low tissue background and faint visualization of the radiotracer in the spleen. Blood clearance of the four99mTc-sulfur colloids was determined in rabbits. The data obtained indicated that the four hepatoagents exhibit rapid blood clearance but the initial decrease of blood activity curve of kit D was relatively faster than the other three hepatic agents. The biodistribution is similar for the four99mTc-S-colloids but the blood retained higher activity residue using kit A compared with others. The formation of99mTc-sulfur colloid using kits B, D (multistep kits) involves many steps after the addition of99mTcO 4 to the reagent. These procedures are time consuming, required facilities at the medical institutions and give rise to the radiation exposure. While single step kits A and C have the same diagnostic value, the use of kit C allows a reduction of absorbed radiation, which may be useful in the liver exploration in children.

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Abstract  

Labelling of DTPA bicyclic anhydride coupled antibodies were investigated by determining the effect of DTPA: antibodies, DTPA: Sn molar ratios, pH, dimer and polymer formation of antibodies coupled with DTPA, using three different radionuclides, [111In,90Y and99mTc]. Analyses were performed with by Whatman No. 1 paper strips. Under optimal conditions we have achieved specific activities of111In or90Y labelled antibodies of about 37 kBq/1 g for IgG coupled with about 2 DTPA groups per molecule and protein concentration of 15 mg/ml.

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