Authors:H. Khoshro, M. Bihamta, M. Hassanii, M. Omidi, and M. Aghaei
Low molecular weight glutenin subunits are important components of wheat seed storage protein, and play a significant in determining the end-use quality characteristics of wheat varieties. Allelic variation of the LMW-GS is associated with the significant differences of dough quality in bread and durum wheat, and has been widely evaluated at protein level in wheat and its relatives. In this study seven specific primers, specifically amplify genes located at the
loci, were employed to assay the length variation of LMW-GS genes in the A and D-genomes of diploid wheats. A total of 86 accessions of diploid wheats, including 10 accessions of
and 76 accessions of
, were investigated. Seven alleles were detected in accessions of
locus) by two pairs of specific primers and eighteen alleles were detected in accessions of
locus) by five pairs of specific primers. A higher level of allelic variation of LMW-GS was found in accession
with Nei’s genetic variation index (H) of 0.82 and 0.92, respectively. This allelic variation could be used as valuable source for the enrichment of genetic variations and the alteration of flour-processing properties of the cultivated wheat.
Authors:M. Naghavi, M. Ranjbar, A. Zali, M. Aghaei, M. Mardi, and S. Pirseyedi
Simple sequence repeat (SSR) DNA markers were used to characterize the genetic diversity in 70 accessions of
from Iran as well as to determine relationships among these accessions with 9 accessions of
) and 5
landraces. All twenty SSR primer pairs were polymorphic and identified a total number of 149 alleles corresponding to an average of 7.5 alleles per locus. The highest and lowest PIC values were obtained in subsp.
accessions, respectively. Data obtained were used to estimate genetic similarity using the Dice coefficient, and dendrogram was constructed using the UPGMA method. The dendrogram separated the 84 accessions into two main groups. All species grouped according to their genomes. A good level of genetic diversity was observed in the accessions of
, even in geographically close regions, which can be used in the broadening of the genetic base of bread wheat. In addition,
were clustered further away from
, confirming probably chromosomal rearrangements in the Dgenome of
during the processes of evolution.
Authors:A. Nilchi, M. Ghanadi Maragheh, A. Khanchi, M. Farajzadeh, and A. Aghaei
The preparation of novel types of inorganic ion-exchangers, titanium and zirconium phosphates and their ion-exchange properties towards strontium ions, are reported and discussed. Ti(HPO4)2·2H2O is shown to be very stable to hydrolysis and to have high exchange capacity in strongly acid medium. In the case of zirconium phosphate, the titration curves with alkaline earth metal hydroxides are strongly affected by hydrolysis of the exchanger and precipitation of insoluble phosphate. The degree of conversion of the exchanger at which phosphate precipitation begins was found to be 80% for Sr2+. The comparison of Ti(HPO4)2·2H2O with the corresponding zirconium phosphate dihydrated phase suggests that the former possesses a lattice structure different from that reported for the monohydrated exchanger.