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  • Author or Editor: M. Al-Janabi x
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Abstract  

Tin-indium generator systems were made with commercial hydrated zirconium oxide, silica gel and hydrated zirconium oxide prepared by the AMPHLETT method. The adsorption capacity of tin has been determined by both spectrophotometric analysis and gammaspectrometry. Zirconium break-through has been determined and compared with the literature values. The dependence of the adsorption capacity on the particle size has been investigated. The effect of autoclaving on the generator systems has been examined.

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Abstract  

Human serum albumin microspheres were labelled with99mTc as a single step kit with radiochemical yields higher than 95%. With respect to the organ distribution in mice, the per cent of injected dose in liver was 78%.

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Abstract  

Several chromatographic methods have been used for determining the radiochemical purity of99mTc-phytate. Good separation of99mTc-phytate from radiochemical impurities was performed using gel column chromatography packed with Sephadex G-10. Reduced99mTc-complexes and99mTc-phytate were not separated by paper and thin layer chromatography.

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Abstract  

Paper chromatography and Gel Chromatography Column Scanning technique (GCS) have been applied for the separation of indium fractions in113mIn-radiopharmaceuticals. By these techniques the percentage of ionic indium,113mIn-colloid and113mIn-compound have been determined. The resolution efficiency of the gels was found to be significantly influenced by the gel type media and the pH of the eluent. The results obtained from the GCS-profiles indicated that the Sephadex G-50 Fine was the best and can be routinely used in the radiochemical quality control of the113mIn-phytate. Good separation of113mIn-colloids,113mIn-microaggregate and113mIn-phytate from carrier-free113mIn-eluate was performed using Whatman No. 3, previously washed with 0.04N HCl and developed either with 0.9% NaCl (for113mIn-colloid), or 85% methanol (for113mIn-phytate), or phosphate-methanol buffer (for113mIn-microaggregate) as rapid and simple procedure for determination of the radiochemical quality control of indium compound in the forms of radiocolloids.

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Abstract  

Kits containing macroaggregated albumin were prepared and compared with three commercial kits with respect to particle size distribution, radiochemical yield and biodistribution. Our preparation was comparable to the commercial products.

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Abstract  

Sn-alanine kits were prepared in lyophilized form containing 7.02·10–2 M -alanine and 5.5·10–4M stannous chloride dihydrate. The optimal pH value of the preparation was found to be equal to 4.3–5.1. The radiochemical purity and the stability of99mTc-alanine were assessed by gel filtration column scanning techniques (GCS) and thin layer chromatography, and the labelling yield of the complex was higher than 95%. The organ distribution data in mice showed that more than 90% of the injected dose had been accumulated in the liver. However, a negligible amount of radioactivity was detected in the non-target organs. The stability of99mTc-alanine was followed for 5 hours and the Sn-alanine kit was stable for at least 3 months.

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Abstract  

Kits were developed-for the sterile labelling of phytate with99mTc. The effect of molar ratio of phytate to stannous chloride, pH, dilution of the Sn-phytate with99mTc-generator eluate, time of incubation, the shelf life of99mTc-phytate and the storage time of Sn-phytate on the labelling yield of phytate with99mTc was investigated using paper chromatography and gel chromatography column scanning method (GCS). Organ distribution was performed in rabbits and mice. Excellent human liver scans were obtained.

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Abstract  

Correlation between the in vivo distribution and the chemical formulation of99mTc-PYP complex has been studied. We chose mice to evaluate in vivo biodistribution and gel chromatography column scanning technique for radiochemical analysis. The influence of the pH, Sn(II), pyrophosphate concentration and molar ratios of Sn: PYP on the labelling of pyp with99mTc has been investigated in vitro and in vivo. The reaction between99mTc and Sn-PYP was complete within a few minutes. The stability studies were evaluated against dilution. Induced myocardial infarction was evaluated in rats. The clinical evaluation showed excellent definition of sternum and ribs with little blood background activity with normal subjects. Discrete localization of abnormally high activity was shown in the site of recent infarction of the left ventricular myocardium.

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Abstract  

Kits were developed for the preparation of a sterile113mIn colloid as a radiopharmaceutical for liver scanning. 2.5 ml of113mIn sterile generator eluate was added to 0.5 ml of ferric chloride dissolved in 0.04N HCl (40 g/ml). The pH was adjusted by addition of 2 ml of phosphate buffer. The optimal pH for the formation of113mIn colloid was found to be equal to 7.5–8.5. Liver uptake in mice was determined to be 85–90%.

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Abstract  

Higher than 90% of113mIn radioactivity was bound to microaggregates. The liver uptake in mice was (80%) with low lung uptake (1.3%). With respect to99mTc-microaggregated albumin, the radiochemical yield was higher than 95%. The liver uptake in mice was about (80%) with low lung uptake (1.7%). The stability of the microaggregates was followed for two months.

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