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  • Author or Editor: M. Chliyeh x
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The evolution of anthracnose symptoms on the aerial part (leaves, stems and strawberries) of three varieties Fortuna, Camarosa and Festival of strawberry plants inoculated with the conidial suspensions of Colletotrichum acutatum and Colletotrichum gloeosporioides isolates was followed. The severity index and infection coefficients increased in function of time. Seven days after inoculation they were low not exceeding 13.43% and 43.33, but they increased four weeks after inoculation, respectively, to 37.96% and 99 on strawberry plants of the Camarosa variety, 54.44% and 105 on those of Fortuna and 51.12% and 85 on those of Festival. At the sixth week, the severity index and infection coefficients became very high, reaching respectively 100% and 408 on Fortuna plants inoculated with C. gloeosporioides isolate (Coll3) followed by Coll2 (89.28% – 300), Coll1 (86.66% – 378) and Coll4 (80.45% – 198) of C. acutatum species. Similarly, the isolate Coll3 caused fruit rot; the percentage of rotten strawberries was 100% on Fortuna variety, 83.33% on Festival and 70.25% on Camarosa. A positive re-isolation of the tested Colletotrichum isolates has been noted from leaves of strawberry varieties and negative from crowns or the roots. A significant to moderate reduction in fresh and dry weights of the aerial part and roots was noted in inoculated strawberry plants compared to the control.

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Authors: I. El Aymani, S. El Gabardi, M. Artib, M. Chliyeh, K. Selmaoui, A. Ouazzani Touhami, R. Benkirane and A. Douira

The diversity of endomycorrhizal fungi in the rhizosphere of Crocus sativus has been studied at five sites in the Taliouine region (Tinfat), located in Taroudant Province (Morocco), according to the number of years of soil exploitation by Saffron cultivation. In all sites, the roots of Crocus sativus carry structures of endomycorrhizal fungi. Root mycorrhizal frequencies are very high in site 1 (93.33%); site 2 (96.67%); site 4 (90%) and in site 6 (93.33%). In these sites, the spore density is, respectively, 39, 58, 138, 99 spores / 100 g of soil. The frequency of root mycorrhization is lower at the site (76.66%) which also exhibited a spore density of 27 spores / 100 g of soil.

The identification of isolated spores made it possible to note the presence of 36 species belonging to 6 genera: Glomus (15 species), Acaulospora (10 species), Scutellospora (6 species), Gigaspora (2 species), Pacispora (2 species), Entrophospora (1 species). Species such as Glomus clarum, G. etunicatum, G. aggregatum, G. intraradices, Acaulospora laevis, Scutellospora coralloidea, were present in all studied sites.

The greatest richness of MA fungi was registers in the site at four successive years of exploitation by Saffron (24 species), with a Shannon diversity index H ‘= 2.82 which is the highest among all studied sites, followed by the site at six years of occupation by Saffron (21 species), with H ‘= 2.61, while the lowest number of species was recorded in sites of two, three and ten years of exploitation of sol by Saffron, with H ‘= 1.77, respectively; 2.12 and 2.44.

This decrease in endomycorrhizal species richness confirms that Crocus sativus residues are probably the cause. In fact, the prolonged occupation of plots with safrana has an allelopathic effect on mycoflora and on the yield of Saffron.

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Authors: N. El Hazzat, M. Artib, J. Touati, M. Chliyeh, K. Selmaoui, A. Ouazzani Touhami, R. Benkirane and A. Douira

The endomycorrhizal fungi diversity in the rhizosphere of chickpea (Cicer arietinum L.) and the evaluation of root mycorrhizal level were studied in six regions of Morocco: Tahla, Sefrou, Souk Larbae, Souk Tlat, Ouazzane and Jarf Melha. All chickpea roots are carrying endomycorrhizal structures. Root mycorrhizal parameters varied from one site to another, and the highest frequency and intensity of mycorrhization was recorded in the roots of chickpea plants at the two sites Tahla and Jarf Melha respectively, 83%, 33% and 25.03%. In addition, the highest arbuscular content was also noted in the roots of plants growing in the site of Tahla (22.18%) while the lowest content was noted at the site of Sefrou (2.07%). However, the vesicles were not observed in all the sites.

The highest numbers of endomycorrhizal spores were recorded in the rhizosphere of plants collected in Jarf Melha and Tahla, respectively, 74 and 41 spores / 100 g soil. All spores found in the studied sites are represented by 22 morphotypes belonging to 7 genera: Glomus (13 species), Acaulospora (4 species), Gigaspora (one species), Radekera (one species), Entrophospora (one species), Pacispora (one species), Dentiscutata (one species).

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