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  • Author or Editor: M. El-Banna x
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Summary  

Simpson Violet dye has been exposed to γ-irradiation in non-aqueous solvents, and the color bleaching by irradiation was studied. The color bleaching was related to the applied dose. In another series of experiments, Simpson Violet dye was incorporated in polymethylmethacrylate films for studying the dose-response relationship. Finally, the results were compared with those obtained for externally dyed polymeric films. Most of the systems investigated were found suitable for gamma dose evaluation within a certain dose range.

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Abstract  

In the present work, the radiation-induced color bleaching of Remazol brilliant blue (RBB), Wegocet orange (WO), Methyl green (Me G) and Thioflavine S (Th S) dyes solutions was studied. Solutions of these dyes in different solvents were found to obey Beer’s law within certain concentration levels. The % color bleaching occurring in different dye solutions on using different gamma irradiation doses was determined and the data obtained showed the existence of good linear relationships among them in the four dye systems used. The linear sections lines were used as calibration curves for evaluating unknown gamma irradiation doses. From the obtained results, it was concluded that RBB in water, WO in ethanol, Me G in butanol and Th S in 60% ethanol–water mixture could be used for dose evaluation within the dose ranges 5–25 kGy for RBB, 20–90 kGy for WO, 10–70 kGy for Me G and 5–160 kGy for Th S. The sensitivity of the systems towards gamma radiations has been also reported.

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Fasciated and normal stem segments of Opuntia microdasys, Opuntia cylindrica, Huernia primulina and Euphorbia lactea were collected from the same plant and compared for their anatomy, water relations and genetic variations. Anatomical differences in terms of thickness of cuticle, vascular bundle, xylem and phloem were analyzed in both normal and fasciated stems. The mucilage cells were higher in the fasciated form of Opuntia microdasys than that in the normal form. Water status in terms of total water content (TWC), water deficit and relative water content (RWC) was influenced by fasciation. Genetic variations were tested in normal and fasciated stems using randomly amplified polymorphic DNA (RAPD) fingerprints and SDS-PAGE of soluble protein extracts. SDS-PAGE protein and RAPD analysis confirmed that normal and fasciated tissues were genetically different. Polymerase chain reaction (PCR) yielded different polymorphic banding patterns that were unique to each primer and distinguishable over all samples. The PCR results of normal and fasciated samples were significantly different in cases of primers P1, P2 and P3. These results indicate that occurrence of fasciation in Opuntia microdasys, Opuntia cylindrica, Huernia primulina and Euphorbia lactea is an epigenetic mutation of tissues.

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