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A RFLP approach was used to investigate polymorphism of ω -gliadin genes in Ae. tauschii using a F 2 population from the cross of accessions AUS18913 and CPI110856. A set of 150 F 2 progenies was genotyped by acid polyacrylamide gel electrophoresis (A-PAGE) and only one recombinant line of Gli-D t 1/Gli-D t T1 was observed. Twelve restriction enzymes were initially tested on genomic DNA of the two parents of which four restriction enzymes revealed polymorphism. Of these four, only Dra I was associated with the novel ω -gliadin gene (T 1 ) using a 1,200 bp DNA fragment of a ω -gliadin gene as a gene-specific probe. The ω -gliadin gene (T 1 ) may be of interest for further studies relating storage proteins and wheat bread-making quality.

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Cereal Research Communications
Authors: A. shahnejat Bushehri, A. Salavati, B. yazdi Samadi, M. Hassani, and S. Shahnejat Bushehri

A collection of new and obsolete Iranian bread wheat cultivars were characterized for gliadins using acid polyacrylamide gel electrophoresis (A-PAGE). Extensive polymorphism (H) = 0.734 in gliadin patterns was found. A total of 26 band patterns including 13, 8 and 5 different mobility bands were identified, in the zones of ω-, β + γ- and α-gliadins, respectively. There were a few patterns specific to each region and some were common among all the regions. Patterns of α-gliadin C, β + γ-gliadins A, and ω-gliadins H, C and E patterns were significantly higher in temperate and tropical zones. β + γ-gliadin C and ω-gliadin Q were significantly higher in Caspian-cold regions. Variation was observed in gliadins patterns of cultivars grown in different regions in Iran. Individual cultivars showed unique gliadin fingerprints. There were larger variation in ω- and γ + β-gliadins than in α-gliadins. These results may provide complementary information for relating genetic diversity, and quality characterization of Iranian wheat cultivars.

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