Authors:M. Hassani, M. Naghavi, M. Shariflou, and P. Sharp
A RFLP approach was used to investigate polymorphism of
-gliadin genes in
using a F
population from the cross of accessions AUS18913 and CPI110856. A set of 150 F
progenies was genotyped by acid polyacrylamide gel electrophoresis (A-PAGE) and only one recombinant line of
was observed. Twelve restriction enzymes were initially tested on genomic DNA of the two parents of which four restriction enzymes revealed polymorphism. Of these four, only
I was associated with the novel
-gliadin gene (T
) using a 1,200 bp DNA fragment of a
-gliadin gene as a gene-specific probe. The
-gliadin gene (T
) may be of interest for further studies relating storage proteins and wheat bread-making quality.
Authors:A. shahnejat Bushehri, A. Salavati, B. yazdi Samadi, M. Hassani, and S. Shahnejat Bushehri
A collection of new and obsolete Iranian bread wheat cultivars were characterized for gliadins using acid polyacrylamide gel electrophoresis (A-PAGE). Extensive polymorphism (H) = 0.734 in gliadin patterns was found. A total of 26 band patterns including 13, 8 and 5 different mobility bands were identified, in the zones of ω-, β + γ- and α-gliadins, respectively. There were a few patterns specific to each region and some were common among all the regions. Patterns of α-gliadin C, β + γ-gliadins A, and ω-gliadins H, C and E patterns were significantly higher in temperate and tropical zones. β + γ-gliadin C and ω-gliadin Q were significantly higher in Caspian-cold regions. Variation was observed in gliadins patterns of cultivars grown in different regions in Iran. Individual cultivars showed unique gliadin fingerprints. There were larger variation in ω- and γ + β-gliadins than in α-gliadins. These results may provide complementary information for relating genetic diversity, and quality characterization of Iranian wheat cultivars.