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  • Author or Editor: M. Jassim x
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Abstract  

Kits were developed for labeling sulphur microcolloids with99mTc. The microcolloids were prepared to get the desired particle size. The stannous chloride was treated with sulphide ions released from thioacetamide in the presence of carboxymethyl cellulose and the pH of the reaction was adjusted to 3.0. The contents of single reaction vial were reacted with99mTc, the radiochemical yield was higher than 95%. Sulphur-microcolloid kits were stable and the stability was followed for 6 hours. The freeze-dried kits were followed more than three months after production and were found stable. Bone marrow uptake in rabbits was determined to be about 36%. The preparation of99mTc-sulphur microcolloid is performed in single step process and axellent node scintigraphy was obtained using experimental animal.

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Abstract  

Correlation between the in vivo distribution and the chemical formulation of99mTc-PYP complex has been studied. We chose mice to evaluate in vivo biodistribution and gel chromatography column scanning technique for radiochemical analysis. The influence of the pH, Sn(II), pyrophosphate concentration and molar ratios of Sn: PYP on the labelling of pyp with99mTc has been investigated in vitro and in vivo. The reaction between99mTc and Sn-PYP was complete within a few minutes. The stability studies were evaluated against dilution. Induced myocardial infarction was evaluated in rats. The clinical evaluation showed excellent definition of sternum and ribs with little blood background activity with normal subjects. Discrete localization of abnormally high activity was shown in the site of recent infarction of the left ventricular myocardium.

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Abstract  

The formulation of a freeze-dried sulphide colloid kit, to be directly labelled with technetium is based on the preparation of tin/II/ sulphide precolloid, stabilized with carboxy methyl cellulose. The formation kinetics of the labelled colloid assessed by GCS-method showing a fast reduction of99mTc-pertechnetate, forming reduced99mTc-colloid with subsequent build-up of the labelled sulphur colloid. This is assumed to be due to the formation of99mTc/V/ to be bound to sulphide precolloid of the kit. The blood clearance data of the labelled colloid in rabbits showed two exponential disappearance phases of radioactivity from blood with biological half times of 3 and 67 min. The results of organ distribution in mice show that more than 85% of the administered activity is accumulated in the liver indicating a high colloidal yield of optimal particle size. The dynamic studies of the labelled colloid achieved in normal subjects using gamma camera indicate a rapid blood clearance and high liver uptake with low surrounding tissue background.

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