Authors:D. Ladhalakshmi, M. Ramiah, T. Ganapathy, M. Krishna Reddy, S. E. Khabbaz, S. Karunakaran and A. Kamalakannan
A new necrosis viral disease was observed in blackgram, showed brown necrotic rings along with veinal and stem necrosis. The virus was mechanically inoculated on the local lesion host, cowpea cv. 152 and maintained in the local lesion host throughout the study. Yield studies under pot culture experiment showed 10- to 30-day-old plants were highly susceptible and the yield became almost nil. By using Transmission Electron Microscope (TEM) and indirect Direct Antigen Coated-Enzyme Linked Immuno-Sorbent Assay (DAC-ELISA) studies the virus was identified as Tobacco streak virus (TSV). The ultraviolet absorbance of the purified virus was measured and the ratio of A260/A280 was determined as 1.41. Polyclonal antiserum was raised against blackgram necrosis virus in New Zealand white rabbit and the titre value was determined as 1: 200. Direct antigen coating-ELISA was used to detect the virus concentration in various plant parts and stem portion recorded maximum virus concentration. TSV in blackgram was not transmitted through seeds.
Authors:M. Vamsi Krishna, R. N. Dash, B. Jalachandra Reddy, P. Venugopal, P. Sandeep and G. Madhavi
An isocratic ion-pair reversed phase high-performance liquid chromatography-ultraviolet (RP-HPLC-UV) method for analysis of eberconazole nitrate in bulk and in pharmaceutical dosage forms has been developed and validated. Best separation was achieved on Lichrospher C18 column (250 mm × 4.6 mm, 5 μm) using a mobile phase of 10 mM potassium dihydrogen phosphate containing 10 mM tetra-butyl ammonium hydroxide (pH adjusted to 2.8 with ortho phosphoric acid) and methanol (75:25, v/v) at a flow rate of 1.0 mL min−1. UV detection was performed at 220 nm. The method was validated for specificity, linearity, precision, accuracy, limit of detection, limit of quantification, robustness, and solution stability. The calibration plot was linear over the concentration range of 10–80 μg mL−1 (r2 = 0.999) and the limits of detection and quantification were 0.3 and 0.9 μg mL−1, respectively. Intra-day and inter-day precisions were 1.13% and 1.67%, respectively. Experimental design was employed to optimize the method. The method was successfully used for analysis of eberconazole nitrate in commercially available cream (Ebernet).
Authors:G. Murthy, M. Sivaiah, S. Kumar, V. Reddy, R. Krishna and S. Lakshminarayana
The applicability of zirconium phosphate-ammonium molybdophosphate (ZrP-AMP) for the efficient removal of cesium from aqueous
acidic solutions by adsorption has been investigated. The adsorption data analysis was carried out using the Freundlich, Dubinin-Raduskevich
(D-R) and Langmuir isotherms for the uptake of Cs in the initial concentration range of 3.75.10-5-7.52.10-3 mol.dm-3 on the ZrP-AMP exchanger from nitric acid medium. The mean free energy (E) values for the adsorption of Cs were obtained from the D-R isotherm. Equilibrium adsorption values at different temperatures
have been utilized to evaluate the change in enthalpy, entropy and free energy (ΔH°, ΔS°, ΔG°). The adsorption of cesium on the ZrP-AMP exchanger was found to be endothermic.