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Abstract  

Six elements in several organs of mice fed with Zn deficient diet (Zn-def. mice) and those fed with control diet (control mice) were analyzed by INAA. Zinc concentrations in the organs of Zn-def. mice were not distinctly lower than those of control mice except for bone and pancreas. However, Ce content increased significantly in all organs of Zn-def. mice compared with control mice, indicating the partial substitution of Co with Zn in metal proteins or other materials for the Zn-def. mice.

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Abstract  

Use of an enriched156Dy isotope as an activable yield tracer for the determination of lanthanoid contents in various biological reference materials has been proposed. The method consists of preconcentration of the lanthanoid in the156Dy doped samples followed by neutron irradiation and further chemical purification steps. The chemical behaviour of lanthanoid elements in the whole procedure was found, in separate runs, similar to that of the added156Dy within experimental errors. Simple purification steps after irradiation allow the measurement of relatively short-lived nuclides and diminish the radiation dose received during the chemical treatment. The present results for orchard leaves (NBS SRM 1571) are generally in good agreement with the previously reported data. Some new data are obtained for other biological reference materials.

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Abstract  

The stability constants (β 1) of iodide ion-pairs of trivalent f-block element ions (lanthanoids Ce, Eu, Gd, Tb and Tm, and actinoid Am) were determined in the vicinity of pH 2.5 of mixed methanol/water solvent solutions of an ionic strength of 1.00 mol·dm−3 at 298±1 K. The values were less than 2. From the variation in distance between Eu3+ and I, which was calculated using a Born-type equation for Gibbs’ free energy derived from β 1(Eu), the Eu3+-I interaction was shown to be solvent-shared ion-pair formation when the mole fraction of methanol (X MeOH)≤0.40. In contrast, it was suggested that the interaction of Am3+-I changed from solvent-separated ion-pair to solvent-shared ion-pair with increasing X MeOH when X MeOH≤0.10, but remained as solvent-shared ion-pair in the range 0.16≤X MeOH≤0.40. Furthermore, β 2(Am) was measured in the range 0.31≤X MeOH≤0.40. It was also shown that the β 1 values of lanthanoids at X MeOH = 0.40, except for that of Gd(III), decreased with increasing atomic number.

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Abstract  

The formation constants of thiocyanate complexes of Eu(III) and Am(III) in trace concentrations were investigated in mixed solvent (CH3OH+H2O) solutions of different ionic strength. Furthermore, in paper electrophoresis, the moving velocities of the species of Eu(III) and Am(III) were investigated in 1.1M (H, Na)(SCN, ClO4) mixed solvent (CH3OH-H2O) solutions. The results showed that the difference between the velocities of Eu(III) and Am(III) is explained by the difference of the mean charges calculated by the formation constants of thiocyanate complexes of Eu(III) and Am(III) in the solution.

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Abstract  

Instrumental neutron activation analysis (INAA), and inductively coupled plasma mass spectroscopy (ICP-MS) were applied to the determination of major elements and rare earth elements in Japanese Standard Soil Materials (NDG-1 to -8). Eight major elements, Al, Fe, Ti, Ca, Mg, Mn, K, and Na were determined by INAA. A comparison of the data for rare earth elements obtained by INAA and ICP-MS shows that the data of the contents determined by the two different analytical methods are in fairly good agreement with each other.

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Abstract  

The behavior of Zn and other trace elements in mice fed with Zn-deficient diet during the growing period (Zn-dcf. mice) was investigated. A correlation between Zn concentration and Co concentration was found in the liver of Zn-def. mice. Two types of correlation between Zn and Mn concentrations were also recognised for the same livers. These facts suggest that Zn and Co, and also Mn are antagonistic, and that Co- and Mn-substitution for Zn-proteins and other Zn-bound compounds may occur, and/or the substituted compounds may be synthesized in the liver of Zn-def. mice.

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Abstract  

Eleven elements in ten organs and tissues of mice fed with Zn-deficient diet (Zn-def. mice) and those fed with control diet (control mice) were determined by INAA. Zinc concentrations in the organs of Zn-def. mice were not distinctly lower than those of control mice except for bone and pancreas, as similar to the predecessors' reported results. However, the Co content increased significantly in all the organs and tissues of Zn-def. mice compared with control mice. The organs and tissues observed were histologicaly normal and no typical symptoms of Zn deficiency disease were recognised. The results suggest that the change of Co-concentration may be regarded as a mark of the prestage for a Zn deficiency disease.

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Journal of Radioanalytical and Nuclear Chemistry
Authors: J. Kamishima, T. Ogi, R. Minayoshi, N. Kinugawa, H. Suganuma, M. Noguchi, K. Ishikawa, and M. Yanaga

Abstract  

Eleven elements in hepatocyte of mice fed with zinc deficient diet and those fed with control diet were determined by INAA. Zinc concentration, especially, in cytosolic fraction of zinc deficient mice was lower than that of control ones. On the other hand, cobalt concentrations in all fractions of zinc deficient mice were higher than those of control ones. However, when SDS-PAGE was performed for cytosolic fraction of hepatic cells, the positions and the number of the protein bands on gel developed for zinc deficient mice were not different from those for control ones.

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Abstract  

Multitracer, a new tracer technique developed by us, can analyze metabolism and behavior of different elements in the living bodies simultaneously. We were able to reproduce completely the results on the behavior of various elements obtained by individual experiments of our precursors only by a single run of this technique. At the same time, we could also show the behavior and interaction of many other trace elements which past researchers did not pay attention to. For example, our results on the behavior of rare-earth elements suggested that there is a correlation between their uptakes and ion radii. We also discovered that affinities of platinum and iridium for their binding proteins were changed, the latter being increased, in the case of zinc deficiency. In this article, we discuss the usefulness of the multitracer technique in the studies of trace elements in living bodies.

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Journal of Radioanalytical and Nuclear Chemistry
Authors: T. Ohyama, M. Yanaga, T. Yoshida, H. Maetsu, M. Noguchi, H. Suganuma, T. Omori, R. Hirunuma, and S. Enomoto

Abstract  

A multitracer technique was used to obtain uptake rates of essential trace elements in various organs and tissues in Zn-deficient mice. A multitracer solution, containing more than 20 radioisotopes, was injected intraperitoneally into Zn-deficient state mice and control ones. Uptake rates of the radioisotopes were compared with concentrations of trace elements determined by instrumental neutron activation analysis (INAA) in order to study a specific metabolism of Zn and other essential trace elements, such as Mn, Co, Se, Rb, and Sr. The result suggests that Zn is supplied from bone to other organs and tissues and an increase in Co concentration in all organs and tissues depends on its chemical form, under the Z-deficient state.

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