Authors:H. Essafi, N. Trabelsi, C. Benincasa, A. Tamaalli, E. Perri and M. Zarrouk
The aim of the present study was to investigate the biological activities of Tunisian olive leaf extracts and to correlate these activities to their phytochemical composition. The phenolic profile of four Tunisian autochthonous cultivars Chemlali, Sayali, Neb jmel, and Meski was determined using LC/MS-MS. The antioxidant activity of olive leaf extracts was evaluated using DPPH test. The antiproliferative effect was also investigated using MTT assay. The phytochemical screening showed that phenolic content and phenolic class repartition were significantly affected by olive leaf cultivars. Twenty-one components were identified and oleurpein, luteolin 4-glucoside, luteolin 7-glucoside, and apigenin 7-glucoside were the major phenolic components. Among all extracts, Sayali exhibited the strongest antioxidant and antiproliferative activities (IC50 41.36 µg ml−1 / EC50 147.11 µg ml−1). The MTT result showed that olive leaf extract reduced MCF-7 cell viability in a dose-dependent manner. Western blot analysis demonstrated that olive leaf extracts exhibited antiproliferative activity through apoptosis induction.
Authors:P. V. Drion, A. Zarrouk, J. Sulon, O. Szenci, J. F. Beckers, Zs. Perényi, B. Remy and J. M. Garbayo
The Pregnancy Associated Glycoproteins (PAGs) presented in this paper are largely expressed in the ruminant placenta. These proteins are classified as probably inactive members of the aspartic proteinase family. Pepsinogen, renin, cathepsin E & D and chymosine are typical members of this family, characterised by the presence of aspartic acids boarding the recognition sites. Secreted in the peripheral blood of the pregnant female from early pregnancy, these proteins can be used in serological tests for establishing different diagnoses. In the veterinary practice, these diagnoses are useful for both pregnancy confirmation and follow-up of trophoblastic function. The first aspect can help breeders in the management of reproduction, while the second one more specifically concerns clinicians and researchers wishing to establish a differential diagnosis of pathologic conditions affecting pregnancy.