Authors:Anatolii Romaniuk, Anna Borisivna Korobchanska, Yevhen Kuzenko and Mykola Lyndin
In the experiment on the 36 mature rats, the peculiarities of morphological disorders in the lower jaw of rats under the influence of salts of chromium, lead, zinc, iron, copper, and manganese are showed. Profound morphological changes in bone and cartilaginous tissues of lower jaw are followed by disorders of bone mineral content. The importance of the S100 protein in morphological changes in bone and cartilaginous tissues under the influence of heavy metal salts is shown.
Authors:Anatolii Romaniuk, Mykola Lyndin, Roman Moskalenko, Yevhen Kuzenko, Oksana Gladchenko and Yuliia Lyndina
Materials and Methods: Chemical composition was studied with the help of the scanning electron microscope with energy-dispersion spectrometer. Immunohistochemical reaction showed the p53 and Ki-67 receptors expression. The study of DNA fragmentation was performed in agarose gel. Results: There was an interrelation between the accumulations of the trace elements with the degree of cancer malignancy. There were 85% of cases with positive reaction to Ki-67 and 40% cases with positive reaction to p53. We found a moderate correlation between the accumulation of microelements in the breast cancer tissue and the level of proliferative activity. We noted the combination of the increase of DNA fragmentation with the expression of p53 and Ki-67 receptors. Conclusions: The trace elements can cause the initiation and the progression of the tumorous growth, which is expressed in the increased proliferation of tumor cells. This leads to the destabilization of the genetic material which can be expressed in the synthesis of mutant p53 protein. Finally, it leads to the block of apoptosis and regulatory effects of cells. This can cause the tumor progression and the destabilization of the genome, which is reflected in the increased DNA fragmentation.
Authors:Anatolii Romaniuk, Yuliia Lyndina, Vladyslav Sikora, Mykola Lyndin, Ludmyla Karpenko, Oksana Gladchenko and Igor Masalitin
This article is devoted to the investigation of the structural features of the bone marrow of mature rats.
Materials and methods
The investigation of the structural features of the bone marrow was performed on the femurs of the mature male rats. General structure of the organ was studied with hematoxylin–eosin and Van Gieson staining of samples. Certain features of the bone marrow structure were studied using immunohistochemical method (CD3, CD79α, S100, myeloperoxidase, and cyclin D1).
We can state that stromal–parenchymal structure is typical for the bone marrow of rats as for any other organ. The stromal component is presented with bone tissue (48.8 ± 3.3% at epiphyses), the net of blood vessels (18.7 ± 2.1%), fat tissue (11 ± 2%), fibrous tissue (0.7 ± 0.2%), and the network of reticular fibers. Hematopoietic tissue covers 20.9 ± 3.7% at the femoral epiphyses and 69.6 ± 2.2% at diaphysis. Among these tissues, myelopoiesis occupies 74.2 ± 4.7%, erythropoiesis – 24.3 ± 4.7%, and lymphopoiesis – less than 5%. Megalokaryocytes take 0.1–0.3%.
Considering the lack of significant anatomical, morphological, and histological differences of red bone marrow of rats and humans, we can state that hematopoiesis in rats takes place on the basis of the same principles as in humans, although it has certain mechanisms.
Authors:Anatolii Romaniuk, Nataliya Gyryavenko, Mykola Lyndin, Sofiia Romaniuk, Miroslav Starkiv and Galina Slobodyan
The objective of this study was to give an overview of a rare case of tuberculosis of the left fallopian tube in postmenopausal women. It is known that the isolated tuberculous salpingitis without spreading to the endometrium is extremely rare case. In our case, it simulated a cancer of appendages at the late stages that were proved by the increased level of CA-125. This fact as well as the macroscopic image of the left fallopian tube was incorrectly considered by the clinicians as a malignant neoplasm of fallopian tube. However, after pathomorphological examination of postoperative samples during histological study, the main method of verification of the diagnosis, revealed the specific granulomas in the samples that indicate the tubercular inflammation. It was considered by clinical and laboratory as data secondary focus.
Authors:Anatolii Romaniuk, Vladyslav Sikora, Mykola Lyndin, Vladyslav Smiyanov, Volodymyr Sikora, Yulia Lyndina, Artem Piddubnyi, Nataliya Gyryavenko and Anna Korobchanska
This study is aimed to the investigation of the features of morphological changes in the urinary bladder of mature rats.
Received results shown that the experimental group of rats that had the intake of heavy metal salts (HMS) mixture during 30 and 90 days were observed histological and immunohistochemical changes in all structures of the urinary bladder. Depending on the period of influence of heavy metal ions on the wall of the organ, the pathological changes developed in cellular layers of the wall can lead to degenerative and later to atrophic and sclerotic changes.
This study demonstrates that high concentrations of HMS can significantly influence the body. The histological and immunohistochemical studies showed that the influence of the HMS combination leads to deep morphological changes in all structures of the urinary bladder. These changes depend on the period of intake of HMS. Analysis of the obtained results demonstrates the dependence of expression of morphological changes in the urinary bladder on the experiment duration. The final result of these changes may lead to the disorders of bladder’s functions.
The purpose of this paper is to describe the research results of the morphological structure of white laboratory rats’ tongue at the macro-, micro-, and ultrastructural levels by scanning, light, and transmission electron microscopy.
Our results show that the tongue of these rats has a number of unique morphological features that are different from the tongue of other rodents consequently to allow identifying their species-specific features.
Our findings have shown the features of the tongue structure of white laboratory rats at micro-, macro-, and ultrascopic levels. The data analysis revealed that mucous membrane of the tongue contains a large number of papillae, such as fungiform, filiform, foliate, vallate, and multifilamentary papillae. Each has a different shape, size, and location. The tongue’s morphological feature consists of three types of filiform papillae, well-developed foliate and multifilamentary papillae, as well as one large and similar smaller circumvallate papillae. The muscle of the tongue contains a large number of mitochondria of different shapes and sizes. However, we have received data for a complete picture of structure of this organ that will be useful in further experimental and morphological studies of the white laboratory rats.
Authors:Olexandr Smiyan, Mykola Lyndin, Oksana Romaniuk, Vladyslav Sikora, Artem Piddubnyi, Alla Yurchenko, Anna Korobchanska, Iryna Tarasova, Natalia Hyryavenko, Kateryna Sikora and Anatolii Romaniuk
Acute lymphoblastic leukemia (ALL) in pregnant women is rare experience, but it can complicate the gestation by increasing the risk of miscarriage and premature birth. However, the adequate carrying of the pregnancy is possible for women who suffered from leukemia in childhood and achieved the remission during the treatment. Furthermore, there are some facts about the possibility of immunosuppression in children whose parents suffer from various immunodeficiency disorders, including ALL. This clinical case demonstrates the importance of correct diagnostics in order to reveal the congenital pathologies of the immune system in children, whose parents suffered from lymphocytic leukemia, even in case of full clinical and laboratory remission for a significant period of time. In the hospital, the thread metric approach was used for sepsis diagnostics. Conducted treatment was ineffective due to the inadequate immune response in the child and lack of the targeted adjusted measures to immunodeficiency disorder. The present case demonstrates the congenital T-cells immunodeficiency in a child who was complicated by the development of acute ulceronecrotic enterocolitis after vaccination. The treatment that was targeted mainly at the agent eradication did not give the desired results due to non-responsiveness of the immune system of the child.
Authors:Alexey Tymoshenko, Gennadii Tkach, Vitalii Sikora, Valentina Bumeister, Ihor Shpetnyi, Mykola Lyndin, Olena Maksymova and Anna Maslenko
The article is devoted to study the structural changes in the skeletal muscles caused by heavy metal salts.
Materials and methods
The study was conducted on 72 mature male rats. The experimental groups were given to drink water with combinations of heavy metal salts for one, two and three months. This type of water is typical for the water basins in the northern districts of the Sumy region. The study of morphological changes in the striated muscles was concluded using light and scanning electron microscopy.
The data analysis revealed that a prolonged duration of negative factor could intensify sclerotic and edematous processes. The structure of muscle fibers was destroyed, nuclei were deformed and placed irregularly, and many petechial hemorrhages occurred. Besides, cross-striation was irregular, I and A bands were deformed and destroyed, H band was hardly visualized. The inner mitochondrial membrane and cristae become deformed. The symplastic nuclei were placed irregularly within sarcoplasm. Besides, they were swollen. Against swollen and enlarged symplastic nuclei, pyknotic nuclei were also found. The structures of sarcoplasmic reticulum were mainly dilated with deformed and ruptured areas.
Our study approves that high concentrations of heavy metal salts have a destructive influence on the skeletal striated muscles.