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  • Author or Editor: N. Bakardjieva x
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Maize dwarf mosaic is the most widespread virus disease affecting corn production in Hungary and Bulgaria. Samples from virus infected maize were collected from different part of Bulgaria and employed test plants, ELISA serological method and RT-PCR in order to identify the viral pathogen. Maize dwarf mosaic virus (MDMV) was detected in all tested samples. For further investigation three MDMV isolates were selected and cloned. Cloned cDNAs representing the coat protein gene of the virus have been sequenced. The coat protein genes of Bulgarian and Hungarian isolates of MDMV were compared. The nucleotide sequence identity and amino acid sequence similarity of the coat protein region varied from 88% to 99.1% and from 95.1% to 99.6%, respectively. The N-terminal region of coat protein was compared with other members SCMV subgroup and phylogenetic tree was constructed.

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Barley-infecting isolates of WDV were collected in the field of near Sofia. The complete genomes of two isolates were amplified by PCR, cloned into pGEM-T plasmid and sequenced. The two clones were the same size and showed complete homology. The WDV-Bg17 clone was compared with Barley dwarf virus, Oat dwarf virus and Wheat dwarf virus isolates. Based on DNA sequences WDV-Bg17 isolate shows high homology (95–97%) to Barley dwarf virus isolates and differs from Oat dwarf virus (71% homology) and Wheat dwarf virus (85% homology).

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