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A simple and reliable preparative high-performance liquid chromatographic technique for isolation of a bioactive flavone diglycoside from and extract of Cuminum cyminum seeds

Acta Chromatographica
Volume/Issue:
Volume 21: Issue 3
DOI:
https://doi.org/10.1556/achrom.21.2009.3.12
Authors:
N. K. Satti
,
M. Amina
,
P. Dutt
,
V. K. Sharma
,
P. Sharma
,
I. Khan
,
B. D. Gupta
,
K. A. Suri
,
S. C. Sharma
,
R. K. Johri
, and
S. N. Sharma

Summary

In this paper we describe a sensitive and reproducible reversed-phase high-performance liquid chromatography (HPLC) method with photodiode-array detection for isolation and quantification of the bioactive hydrophilic constituent 7-(1-O-β-d-galacturonide-4′-(1-O-β-d-glucopyranosyl)-3′,4′,5,7-tetrahydroxyflavone, 1, from the seeds of Cuminum cyminum. Compound 1 was separated isocratically on a C18 preparative column, in high purity, after removal of solvents. The purity and identity of the compound were established by use of LC-mass spectrometry and by spectroscopic techniques (1H and 13C NMR). The purity of 1 was also confirmed by HPTLC.

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