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Acta Agronomica Hungarica
Authors:
J. Pauk
,
C. Lantos
,
G. Somogyi
,
P. Vági
,
Z. Ábrahám Táborosi
,
A. Gémes Juhász
,
R. Mihály
,
Z. Kristóf
,
N. Somogyi
, and
Z. Tímár

Spice pepper production has a history of almost 300 years in the southern part of Hungary. In this study the results of two biotechnological improvements are summarized. Anther and isolated microspore culture techniques were improved to release haploid and doubled haploid (DH) lines for spice pepper breeding. Both the anther and isolated microspore culture methods were successfully used in spice pepper haploid production. Microspore culture-derived structures were analysed to identify their different parts. Green plantlets were regenerated from embryos derived from both anther and microspore cultures. Their doubled haploid analogues were integrated into Hungarian spice pepper hybrid seed breeding programmes. One hybrid, Sláger, was released as a new genotype for spice pepper production in 2008 and two hybrid candidates (Délibáb and Bolero) are now being tested in official trials.

Restricted access
Nanopages
Authors:
I. Szendrő
,
K. Erdélyi
,
Zs. Puskás
,
M. Fábián
,
N. Adányi
, and
K. Somogyi

Due to the changes of the refractive indices, the planar optical waveguides are sensitive to the surrounding media, to the adsorbates, etc. on their surface. The sensitivity of such a waveguide layer can be enhanced when its thickness is lowered down to the nanometer range. Such sensors can be successfully operated both in inorganic chemistry and in life sciences as label free biosensors. Principles and some results are demonstrated. Further on, application of transparent conductive oxides for voltammetric measurements in combination with the classical waveguide sensor will be demonstrated. Development and results of a combined system is described and first results with simultaneous measurements are demonstrated. An indium tin oxide nanolayer is deposited and activated on the top of the sensor chip. This electrically conductive oxide layer serves as working electrode in the specially developed electrochemical cuvette. In this work results are presented for simultaneous use of these two methods and for simultaneous measurement of refractive index changes of the waveguiding system and that of electrical current changes. The first basic results are demonstrated using H2O2 and dye solutions, using KCl and TRIS as buffer and transport media.

Open access