Authors:Nevena Popovic, Bernard Fried, and Joseph Sherma
Biomphalaria glabrata snails were infected with Schistosoma mansoni and maintained at different dilutions of artificial ocean water for up to 4 weeks. Glucose and maltose concentration of the digestive gland-gonad complex were analyzed by high-performance thin-layer chromatography at different stages of the infection. B. glabrata snails were divided into three experimental groups: Group A, snails with early prepatent infection (10 days post-infection); Group B, snails with late prepatent infection (22 days post-infection); and Group C, snails with patent infection (45 days post-infection). Infected snails in A were maintained at different salinities for 2 weeks and then necropsied, and their two main simple sugars, i.e., glucose and maltose, were analyzed. Groups B and C contained two subgroups: the first subgoups were analyzed after 2 weeks, and the second after 4 weeks. Controls for these experiments were maintained identically in either deionized water or artificial spring water. Maltose and glucose were extracted from the digestive gland-gonad complex in ethanol-water (70:30). 1-Butanol-glacial acetic acid-diethyl ether-deionized water (27:18:5:3) mobile phase was used to separate sugars on EMD Millipore silica gel preadsorbent plates. Sugars were detected using α-naphthol-sulfuric acid reagent and quantified with a CAMAG TLC Scanner 3 at 515 nm. The obtained data were compared using analysis of variance (ANOVA) single factor statistical analysis. Statistical differences were not found in any sugars in Group A snails. For glucose, a significant difference was found after 4 weeks in both B and C snails. For maltose, a significant difference was found after 4 weeks in B snails and after 2 weeks in C snails. Different salinity levels affect the maltose and glucose concentrations of adult B. glabrata snails infected with S. mansoni.
Authors:Ciara O’sullivan, Nevena Popovic, Bernard Fried, and Joseph Sherma
The purpose of this study was to identify and quantify various neutral and polar lipids in certain organs of mice using high-performance thin-layer chromatography (HPTLC). Four mice infected with Schistosoma mansoni and three control mice were used for this study. At 6 weeks postinfection, the mice were necropsied, and the liver, spleen, and small intestine were removed and prepared for lipid analysis. Lipids were separated on laned, preadsorbant Analtech HPTLC-HLF 20 × 10-cm silica gel plates. Neutral lipids were separated using petroleum ether-diethyl ether-glacial acetic acid (80:20:1) mobile phase and were detected by spraying with 5% ethanolic phosphomolybdic acid detection reagent. Polar lipids were separated with chloroform-methanol-deionized water (65:25:4) mobile phase and detected using 10% cupric sulfate in 8% phosphoric acid reagent. The analyzed neutral lipids were free sterols, free fatty acids, and triacylglycerols. Using HPTLC, no significant differences were found in these lipids between the infected and uninfected mice organs. The polar lipids analyzed were phosphatidylcholine (PC) and phospatidylethanolamine (PE). There was a significantly higher PC content in the liver and small intestine of the uninfected mice compared with that of infected mice.