Authors:M. Saiki, S. Rogero, I. Costa, O. Correa, and O. Higa
Neutron activation analysis has been applied to analyse ear piercing studs manufactured with three different types of metallic materials and also in the analyses of cell culture media in which these studs were immersed. Results obtained in these analyses indicated the release of metal from the studs to the culture media. Zn and Fe were found, respectively, in the extracts of gold coated copper-zinc alloy and stainless steel. These findings were correlated with the results already obtained for studs surface analysis by scanning electron microscopy and for the cytotoxicity tests of culture extracts.
Authors:J. Baptista, D. Vieira, A. Galisteo Júnior, O. Higa, M. Casare, C. Yonamine, P. Caproni, L. Campos, H. de Andrade Júnior, P. Spencer, and N. Nascimento
In this work, the authors investigated the immunological behavior of bothropstoxin-I (BTHX-1), before and after irradiation
process, and also the influence of scavengers substances on protein alterations induced by free radical production. Structural
modifications were investigated by SDS-PAGE in reducing or non-reducing conditions. In vitro cytotoxicity assay was performed
to test average toxic activities of BTHX-I. BALB/c Isogenic mice were immunized with irradiated or non-irradiated (native)
forms of BTHX-I and antibody titers and isotypes were determined by ELISA method. Expression of murine cytokines was analyzed
by using expression data obtained by quantitative real-time PCR (qPCR) assays. The results indicate that irradiation of proteins
leads to significant structural modifications, and also changes the cytokines profile during immunization process, regarding
a suitable approach to new immunogenic production.